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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 8 — Aug. 1, 2013
  • pp: 1434–1441

Fourier phase microscopy with white light

Basanta Bhaduri, Krishnarao Tangella, and Gabriel Popescu  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 8, pp. 1434-1441 (2013)
http://dx.doi.org/10.1364/BOE.4.001434


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Abstract

Laser-based Fourier phase microscopy (FPM) works on the principle of decomposition of an image field in two spatial components that can be controllably shifted in phase with respect to each other. However, due to the coherent illumination, the contrast in phase images is degraded by speckles. In this paper we present FPM with spatially coherent white light (wFPM), which offers high spatial phase sensitivity due to the low temporal coherence and high temporal phase stability due to common path geometry. Further, by using a fast spatial light modulator (SLM) and a fast scientific-grade complementary metal oxide semiconductor (sCMOS) camera, we report imaging at a maximum rate of 12.5 quantitative phase frames per second with 5.5 mega pixels image size. We illustrate the utility of wFPM as a contrast enhancement as well as dynamic phase measurement method by imaging section of benign colonic glands and red blood cell membrane fluctuation.

© 2013 OSA

OCIS Codes
(070.0070) Fourier optics and signal processing : Fourier optics and signal processing
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.0180) Medical optics and biotechnology : Microscopy
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Microscopy

History
Original Manuscript: May 31, 2013
Revised Manuscript: July 21, 2013
Manuscript Accepted: July 22, 2013
Published: July 25, 2013

Virtual Issues
Novel Techniques in Microscopy (2013) Biomedical Optics Express

Citation
Basanta Bhaduri, Krishnarao Tangella, and Gabriel Popescu, "Fourier phase microscopy with white light," Biomed. Opt. Express 4, 1434-1441 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-8-1434


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