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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 8 — Aug. 1, 2013
  • pp: 1486–1493

Hyperspectral fluorescence microfluidic (HFM) microscopy

Giuseppe Di Caprio, Diane Schaak, and Ethan Schonbrun  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 8, pp. 1486-1493 (2013)
http://dx.doi.org/10.1364/BOE.4.001486


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Abstract

We present an imaging system that collects hyperspectral images of cells travelling through a microfluidic channel. Using a single monochrome camera and a linear variable bandpass filter (LVF), the system captures a bright field image and a set of hyperspectral fluorescence images for each cell. While the bandwidth of the LVF is 20 nm, we have demonstrated that we can determine the peak wavelength of a fluorescent object’s emission spectrum with an accuracy of below 3 nm. In addition, we have used this system to capture fluorescence spectra of individual spatially resolved cellular organelles and to spectrally resolve multiple fluorophores in individual cells.

© 2013 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Microscopy

History
Original Manuscript: May 24, 2013
Revised Manuscript: July 15, 2013
Manuscript Accepted: July 15, 2013
Published: July 31, 2013

Citation
Giuseppe Di Caprio, Diane Schaak, and Ethan Schonbrun, "Hyperspectral fluorescence microfluidic (HFM) microscopy," Biomed. Opt. Express 4, 1486-1493 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-8-1486


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