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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 9 — Sep. 1, 2013
  • pp: 1504–1511

Observing single protein binding by optical transmission through a double nanohole aperture in a metal film

Ahmed A. Al Balushi, Ana Zehtabi-Oskuie, and Reuven Gordon  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 9, pp. 1504-1511 (2013)
http://dx.doi.org/10.1364/BOE.4.001504


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Abstract

We experimentally demonstrate protein binding at the single particle level. A double nanohole (DNH) optical trap was used to hold onto a 20 nm biotin-coated polystyrene (PS) particle which subsequently is bound to streptavidin. Biotin-streptavidin binding has been detected by an increase in the optical transmission through the DNH. Similar optical transmission behavior was not observed when streptavidin binding sites where blocked by mixing streptavidin with excess biotin. Furthermore, interaction of non-functionalized PS particles with streptavidin did not induce a change in the optical transmission through the DNH. These results are promising as the DNH trap can make an excellent single molecule resolution sensor which would enable studying biomolecular interactions and dynamics at a single particle/molecule level.

© 2013 OSA

OCIS Codes
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(350.4855) Other areas of optics : Optical tweezers or optical manipulation
(310.6628) Thin films : Subwavelength structures, nanostructures

ToC Category:
Optical Traps, Manipulation, and Tracking

History
Original Manuscript: June 14, 2013
Revised Manuscript: July 26, 2013
Manuscript Accepted: July 26, 2013
Published: August 1, 2013

Virtual Issues
Optical Trapping and Applications (2013) Biomedical Optics Express

Citation
Ahmed A. Al Balushi, Ana Zehtabi-Oskuie, and Reuven Gordon, "Observing single protein binding by optical transmission through a double nanohole aperture in a metal film," Biomed. Opt. Express 4, 1504-1511 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-9-1504


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