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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 9 — Sep. 1, 2013
  • pp: 1548–1558

Simultaneous imaging of two-photon absorption and stimulated Raman scattering by spatial overlap modulation nonlinear optical microscopy

Keisuke Isobe, Hiroyuki Kawano, Akira Suda, Akiko Kumagai, Atsushi Miyawaki, and Katsumi Midorikawa  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 9, pp. 1548-1558 (2013)
http://dx.doi.org/10.1364/BOE.4.001548


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Abstract

Imaging of simultaneous two-photon absorption and stimulated Raman scattering is accomplished by detecting the intensity changes of the two-color pulses simultaneously and the mathematical operations of addition and subtraction. The stimulated Raman scattering is quantitatively separated from the two-photon absorption, generated in a mixed solution in which a glycerin solution is miscible in various proportions with a quantum dot solution. Our technique is applied to simultaneous two-photon absorption and stimulated Raman scattering imaging.

© 2013 OSA

OCIS Codes
(190.4180) Nonlinear optics : Multiphoton processes
(180.4315) Microscopy : Nonlinear microscopy
(180.5655) Microscopy : Raman microscopy

ToC Category:
Microscopy

History
Original Manuscript: June 14, 2013
Revised Manuscript: July 23, 2013
Manuscript Accepted: July 23, 2013
Published: August 7, 2013

Virtual Issues
Novel Techniques in Microscopy (2013) Biomedical Optics Express

Citation
Keisuke Isobe, Hiroyuki Kawano, Akira Suda, Akiko Kumagai, Atsushi Miyawaki, and Katsumi Midorikawa, "Simultaneous imaging of two-photon absorption and stimulated Raman scattering by spatial overlap modulation nonlinear optical microscopy," Biomed. Opt. Express 4, 1548-1558 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-9-1548


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