OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 9 — Sep. 1, 2013
  • pp: 1559–1570

An optical system for detecting 3D high-speed oscillation of a single ultrasound microbubble

Yuan Liu and Baohong Yuan  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 9, pp. 1559-1570 (2013)

View Full Text Article

Enhanced HTML    Acrobat PDF (2956 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



As contrast agents, microbubbles have been playing significant roles in ultrasound imaging. Investigation of microbubble oscillation is crucial for microbubble characterization and detection. Unfortunately, 3-dimensional (3D) observation of microbubble oscillation is challenging and costly because of the bubble size—a few microns in diameter—and the high-speed dynamics under MHz ultrasound pressure waves. In this study, a cost-efficient optical confocal microscopic system combined with a gated and intensified charge-coupled device (ICCD) camera were developed to detect 3D microbubble oscillation. The capability of imaging microbubble high-speed oscillation with much lower costs than with an ultra-fast framing or streak camera system was demonstrated. In addition, microbubble oscillations along both lateral (x and y) and axial (z) directions were demonstrated. Accordingly, this system is an excellent alternative for 3D investigation of microbubble high-speed oscillation, especially when budgets are limited.

© 2013 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.7160) Medical optics and biotechnology : Ultrafast technology
(170.7170) Medical optics and biotechnology : Ultrasound

ToC Category:

Original Manuscript: June 25, 2013
Revised Manuscript: July 31, 2013
Manuscript Accepted: July 31, 2013
Published: August 8, 2013

Virtual Issues
Bio-Optics: Design and Applications (2013) Biomedical Optics Express

Yuan Liu and Baohong Yuan, "An optical system for detecting 3D high-speed oscillation of a single ultrasound microbubble," Biomed. Opt. Express 4, 1559-1570 (2013)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. K. Ferrara, R. Pollard, and M. Borden, “Ultrasound microbubble contrast agents: Fundamentals and application to gene and drug delivery,” Annu. Rev. Biomed. Eng.9(1), 415–447 (2007). [CrossRef] [PubMed]
  2. S. P. Qin, C. F. Caskey, and K. W. Ferrara, “Ultrasound contrast microbubbles in imaging and therapy: physical principles and engineering,” Phys. Med. Biol.54(6), R27–R57 (2009). [CrossRef] [PubMed]
  3. S. Zhao, K. W. Ferrara, and P. A. Dayton, “Asymmetric oscillation of adherent targeted ultrasound contrast agents,” Appl. Phys. Lett.87(13), 134103 (2005). [CrossRef] [PubMed]
  4. H. J. Vos, B. Dollet, M. Versluis, and N. de Jong, “Nonspherical shape oscillations of coated microbubbles in contact with a wall,” Ultrasound Med. Biol.37(6), 935–948 (2011). [CrossRef] [PubMed]
  5. W. Lauterborn and T. Kurz, “Physics of bubble oscillations,” Rep. Prog. Phys.73, 106501 (2010).
  6. S. P. Qin and K. W. Ferrara, “Acoustic response of compliable microvessels containing ultrasound contrast agents,” Phys. Med. Biol.51(20), 5065–5088 (2006). [CrossRef] [PubMed]
  7. N. de Jong, P. J. Frinking, A. Bouakaz, M. Goorden, T. Schourmans, X. Jingping, and F. Mastik, “Optical imaging of contrast agent microbubbles in an ultrasound field with a 100-MHz camera,” Ultrasound Med. Biol.26(3), 487–492 (2000). [CrossRef] [PubMed]
  8. M. J. Hsu, M. Eghtedari, A. P. Goodwin, D. J. Hall, R. F. Mattrey, and S. C. Esener, “Characterization of individual ultrasound microbubble dynamics with a light-scattering system,” J. Biomed. Opt.16(6), 067002 (2011). [CrossRef] [PubMed]
  9. S. Wilhelm, B. Grobler, M. Gluch, and H. Heinz, “Confocal laser scanning microscopy principles,” in Zeiss Jena ( http://zeiss-campus.magnet.fsu.edu/referencelibrary/laserconfocal.html ).
  10. Y. G. Du, H. Jensen, and J. A. Jensen, “Comparison of Simulated and Measured Non-linear Ultrasound Fields,” Proc. SPIE7968, 79680P, 79680P-10 (2011). [CrossRef]
  11. J. E. Chomas, P. Dayton, J. Allen, K. Morgan, and K. W. Ferrara, “Mechanisms of contrast agent destruction,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control48(1), 232–248 (2001). [CrossRef] [PubMed]
  12. N. de Jong, M. Emmer, C. T. Chin, A. Bouakaz, F. Mastik, D. Lohse, and M. Versluis, ““Compression-only” behavior of phospholipid-coated contrast bubbles,” Ultrasound Med. Biol.33(4), 653–656 (2007). [CrossRef] [PubMed]
  13. J. Sijl, M. Overvelde, B. Dollet, V. Garbin, N. de Jong, D. Lohse, and M. Versluis, ““Compression-only” behavior: A second-order nonlinear response of ultrasound contrast agent microbubbles,” J. Acoust. Soc. Am.129(4), 1729–1739 (2011). [CrossRef] [PubMed]
  14. M. Overvelde, V. Garbin, B. Dollet, N. de Jong, D. Lohse, and M. Versluis, “Dynamics of Coated Microbubbles Adherent to a Wall,” Ultrasound Med. Biol.37(9), 1500–1508 (2011). [CrossRef] [PubMed]
  15. A. L. Klibanov, “Preparation of targeted microbubbles: ultrasound contrast agents for molecular imaging,” Med. Biol. Eng. Comput.47(8), 875–882 (2009). [CrossRef] [PubMed]
  16. N. Deshpande, A. Needles, and J. K. Willmann, “Molecular ultrasound imaging: current status and future directions,” Clin. Radiol.65(7), 567–581 (2010). [CrossRef] [PubMed]
  17. J. R. Lindner, “Molecular imaging with contrast ultrasound and targeted microbubbles,” J. Nucl. Cardiol.11(2), 215–221 (2004). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4 Fig. 5

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited