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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 9 — Sep. 1, 2013
  • pp: 1646–1653

Enumerating virus-like particles in an optically concentrated suspension by fluorescence correlation spectroscopy

Yi Hu, Xuanhong Cheng, and H. Daniel Ou-Yang  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 9, pp. 1646-1653 (2013)
http://dx.doi.org/10.1364/BOE.4.001646


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Abstract

Fluorescence correlation spectroscopy (FCS) is one of the most sensitive methods for enumerating low concentration nanoparticles in a suspension. However, biological nanoparticles such as viruses often exist at a concentration much lower than the FCS detection limit. While optically generated trapping potentials are shown to effectively enhance the concentration of nanoparticles, feasibility of FCS for enumerating field-enriched nanoparticles requires understanding of the nanoparticle behavior in the external field. This paper reports an experimental study that combines optical trapping and FCS to examine existing theoretical predictions of particle concentration. Colloidal suspensions of polystyrene (PS) nanospheres and HIV-1 virus-like particles are used as model systems. Optical trapping energies and statistical analysis are used to discuss the applicability of FCS for enumerating nanoparticles in a potential well produced by a force field.

© 2013 OSA

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(290.1990) Scattering : Diffusion
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Traps, Manipulation, and Tracking

History
Original Manuscript: May 24, 2013
Revised Manuscript: July 14, 2013
Manuscript Accepted: July 23, 2013
Published: August 14, 2013

Virtual Issues
Optical Trapping and Applications (2013) Biomedical Optics Express

Citation
Yi Hu, Xuanhong Cheng, and H. Daniel Ou-Yang, "Enumerating virus-like particles in an optically concentrated suspension by fluorescence correlation spectroscopy," Biomed. Opt. Express 4, 1646-1653 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-9-1646


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