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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 9 — Sep. 1, 2013
  • pp: 1654–1661

Aberrations and their correction in light-sheet microscopy: a low-dimensional parametrization

Diwakar Turaga and Timothy E. Holy  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 9, pp. 1654-1661 (2013)
http://dx.doi.org/10.1364/BOE.4.001654


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Abstract

Light sheet microscopy allows rapid imaging of three-dimensional fluorescent samples, using illumination and detection axes that are orthogonal. For imaging large samples, this often forces the objective to be tilted relative to the sample’s surface; for samples that are not precisely matched to the immersion medium index, this tilt introduces aberrations. Here we calculate the nature of these aberrations for a simple tissue model, and show that a low-dimensional parametrization of these aberrations facilitates online correction via a deformable mirror without introduction of beads or other fiducial markers. We use this approach to demonstrate improved image quality in living tissue.

© 2013 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Microscopy

History
Original Manuscript: April 29, 2013
Revised Manuscript: June 20, 2013
Manuscript Accepted: June 30, 2013
Published: August 14, 2013

Citation
Diwakar Turaga and Timothy E. Holy, "Aberrations and their correction in light-sheet microscopy: a low-dimensional parametrization," Biomed. Opt. Express 4, 1654-1661 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-9-1654


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