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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 9 — Sep. 1, 2013
  • pp: 1710–1723

In vivo dark-field imaging of the retinal pigment epithelium cell mosaic

Drew Scoles, Yusufu N. Sulai, and Alfredo Dubra  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 9, pp. 1710-1723 (2013)

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Non-invasive reflectance imaging of the human RPE cell mosaic is demonstrated using a modified confocal adaptive optics scanning light ophthalmoscope (AOSLO). The confocal circular aperture in front of the imaging detector was replaced with a combination of a circular aperture 4 to 16 Airy disks in diameter and an opaque filament, 1 or 3 Airy disks thick. This arrangement reveals the RPE cell mosaic by dramatically attenuating the light backscattered by the photoreceptors. The RPE cell mosaic was visualized in all 7 recruited subjects at multiple retinal locations with varying degrees of contrast and cross-talk from the photoreceptors. Various experimental settings were explored for improving the visualization of the RPE cell boundaries including: pinhole diameter, filament thickness, illumination and imaging pupil apodization, unmatched imaging and illumination focus, wavelength and polarization. None of these offered an obvious path for enhancing image contrast. The demonstrated implementation of dark-field AOSLO imaging using 790 nm light requires low light exposures relative to light safety standards and it is more comfortable for the subject than the traditional autofluorescence RPE imaging with visible light. Both these factors make RPE dark-field imaging appealing for studying mechanisms of eye disease, as well as a clinical tool for screening and monitoring disease progression.

© 2013 OSA

OCIS Codes
(170.4460) Medical optics and biotechnology : Ophthalmic optics and devices
(170.4470) Medical optics and biotechnology : Ophthalmology
(290.4210) Scattering : Multiple scattering
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Ophthalmology Applications

Original Manuscript: July 1, 2013
Revised Manuscript: August 9, 2013
Manuscript Accepted: August 14, 2013
Published: August 23, 2013

Virtual Issues
Bio-Optics: Design and Applications (2013) Biomedical Optics Express

Drew Scoles, Yusufu N. Sulai, and Alfredo Dubra, "In vivo dark-field imaging of the retinal pigment epithelium cell mosaic," Biomed. Opt. Express 4, 1710-1723 (2013)

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