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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 1 — Jan. 1, 2014
  • pp: 1–8

0.5 gigapixel microscopy using a flatbed scanner

Guoan Zheng, Xiaoze Ou, and Changhuei Yang  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 1, pp. 1-8 (2014)
http://dx.doi.org/10.1364/BOE.5.000001


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Abstract

The capability to perform high-resolution, wide field-of-view (FOV) microscopy imaging is highly sought after in biomedical applications. In this paper, we report a wide FOV microscopy system that uses a closed-circuit-television (CCTV) lens for image relay and a flatbed scanner for data acquisition. We show that such an imaging system is capable of capturing a 10 mm × 7.5 mm FOV image with 0.78 µm resolution, resulting in more than 0.5 billion pixels across the entire image. The resolution and field curve of the proposed system were characterized by imaging a USAF resolution target and a hole-array target. To demonstrate its application, 0.5 gigapixel images of histology slides were acquired using this system.

© 2013 Optical Society of America

OCIS Codes
(120.4820) Instrumentation, measurement, and metrology : Optical systems
(170.0180) Medical optics and biotechnology : Microscopy
(170.4730) Medical optics and biotechnology : Optical pathology

ToC Category:
Microscopy

History
Original Manuscript: July 18, 2013
Revised Manuscript: November 10, 2013
Manuscript Accepted: November 24, 2013
Published: December 2, 2013

Virtual Issues
Advances in Optics for Biotechnology, Medicine and Surgery (2013) Biomedical Optics Express

Citation
Guoan Zheng, Xiaoze Ou, and Changhuei Yang, "0.5 gigapixel microscopy using a flatbed scanner," Biomed. Opt. Express 5, 1-8 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-1-1


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