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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 1 — Jan. 1, 2014
  • pp: 183–196

Toward nodal staging of axillary lymph node basins through intradermal administration of fluorescent imaging agents

Funda Meric-Bernstam, John C. Rasmussen, Savitri Krishnamurthy, I-Chih Tan, Banghe Zhu, Jamie L. Wagner, Gildy V. Babiera, Elizabeth A. Mittendorf, and Eva M. Sevick-Muraca  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 1, pp. 183-196 (2014)

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As part of a proof-of-concept study for future delivery of targeted near-infrared fluorescent (NIRF) tracers, we sought to assess the delivery of micrograms of indocyanine green to all the axillary lymph nodes following intraparenchymal breast injections and intradermal arm injections in 20 subjects with advanced breast carcinoma and undergoing complete axillary lymph node dissection. Lymphatic vessels and nodes were assessed in vivo. Ex vivo images demonstrated that 87% of excised lymph nodes, including 81% of tumor-positive lymph nodes, were fluorescent. Future clinical studies using microdose amounts of tumor-targeting NIRF contrast agents may demonstrate improved surgical intervention with reduced morbidity.

© 2013 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(170.0110) Medical optics and biotechnology : Imaging systems
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Optics in Cancer Research

Original Manuscript: August 30, 2013
Revised Manuscript: November 28, 2013
Manuscript Accepted: December 4, 2013
Published: December 13, 2013

Funda Meric-Bernstam, John C. Rasmussen, Savitri Krishnamurthy, I-Chih Tan, Banghe Zhu, Jamie L. Wagner, Gildy V. Babiera, Elizabeth A. Mittendorf, and Eva M. Sevick-Muraca, "Toward nodal staging of axillary lymph node basins through intradermal administration of fluorescent imaging agents," Biomed. Opt. Express 5, 183-196 (2014)

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