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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 1 — Jan. 1, 2014
  • pp: 233–243

Imaging and modeling collagen architecture from the nano to micro scale

Cameron P. Brown, Marie-Andree Houle, Konstantin Popov, Mischa Nicklaus, Charles-Andre Couture, Matthieu Laliberté, Thomas Brabec, Andreas Ruediger, Andrew J. Carr, Andrew J. Price, Harinderjit S. Gill, Lora Ramunno, and Francois Légaré  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 1, pp. 233-243 (2014)

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The collagen meshwork plays a central role in the functioning of a range of tissues including cartilage, tendon, arteries, skin, bone and ligament. Because of its importance in function, it is of considerable interest for studying development, disease and regeneration processes. Here, we have used second harmonic generation (SHG) to image human tissues on the hundreds of micron scale, and developed a numerical model to quantitatively interpret the images in terms of the underlying collagen structure on the tens to hundreds of nanometer scale. Focusing on osteoarthritic changes in cartilage, we have demonstrated that this combination of polarized SHG imaging and numerical modeling can estimate fibril diameter, filling fraction, orientation and bundling. This extends SHG microscopy from a qualitative to quantitative imaging technique, providing a label-free and non-destructive platform for characterizing the extracellular matrix that can expand our understanding of the structural mechanisms in disease.

© 2013 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(190.0190) Nonlinear optics : Nonlinear optics

ToC Category:

Original Manuscript: September 24, 2013
Revised Manuscript: November 18, 2013
Manuscript Accepted: November 18, 2013
Published: December 16, 2013

Cameron P. Brown, Marie-Andree Houle, Konstantin Popov, Mischa Nicklaus, Charles-Andre Couture, Matthieu Laliberté, Thomas Brabec, Andreas Ruediger, Andrew J. Carr, Andrew J. Price, Harinderjit S. Gill, Lora Ramunno, and Francois Légaré, "Imaging and modeling collagen architecture from the nano to micro scale," Biomed. Opt. Express 5, 233-243 (2014)

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