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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 1 — Jan. 1, 2014
  • pp: 312–321

Optical imaging of subacute airway remodeling and adipose stem cell engraftment after airway injury

Yeh-Chan Ahn, Sung Won Kim, Sang Seok Hwang, Yu-Gyeong Chae, Andrew Sungwan Lee, Maan Hong Jung, Bong Kwon Chun, Sang Joon Lee, Eun-Kee Park, and Chulho Oak  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 1, pp. 312-321 (2014)

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Acquired airway injury is frequently caused by endotracheal intubations, long-term tracheostomies, trauma, airway burns, and some systemic diseases. An effective and less invasive technique for both the early assessment and the early interventional treatment of acquired airway stenosis is therefore needed. Optical coherence tomography (OCT) has been proposed to have unique potential for early monitoring from the proliferative epithelium to the cartilage in acute airway injury. Additionally, stem cell therapy using adipose stem cells is being investigated as an option for early interventional treatment in airway and lung injury. Over the past decade, it has become possible to monitor the level of injury using OCT and to track the engraftment of stem cells using stem cell imaging in regenerative tissue. The purpose of this study was to assess the engraftment of exogenous adipose stem cells in injured tracheal epithelium with fluorescent microscopy and to detect and monitor the degree of airway injury in the same tracheal epithelium with OCT. OCT detected thickening of both the epithelium and basement membrane after tracheal scraping. The engraftment of adipose stem cells was successfully detected by fluorescent staining in the regenerative epithelium of injured tracheas. OCT has the potential to be a high-resolution imaging modality capable of detecting airway injury in combination with stem cell imaging in the same tracheal mucosa.

© 2013 Optical Society of America

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Optical Coherence Tomography

Original Manuscript: October 11, 2013
Revised Manuscript: December 7, 2013
Manuscript Accepted: December 12, 2013
Published: December 20, 2013

Yeh-Chan Ahn, Sung Won Kim, Sang Seok Hwang, Yu-Gyeong Chae, Andrew Sungwan Lee, Maan Hong Jung, Bong Kwon Chun, Sang Joon Lee, Eun-Kee Park, and Chulho Oak, "Optical imaging of subacute airway remodeling and adipose stem cell engraftment after airway injury," Biomed. Opt. Express 5, 312-321 (2014)

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