OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 1 — Jan. 1, 2014
  • pp: 312–321

Optical imaging of subacute airway remodeling and adipose stem cell engraftment after airway injury

Yeh-Chan Ahn, Sung Won Kim, Sang Seok Hwang, Yu-Gyeong Chae, Andrew Sungwan Lee, Maan Hong Jung, Bong Kwon Chun, Sang Joon Lee, Eun-Kee Park, and Chulho Oak  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 1, pp. 312-321 (2014)
http://dx.doi.org/10.1364/BOE.5.000312


View Full Text Article

Enhanced HTML    Acrobat PDF (2380 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Acquired airway injury is frequently caused by endotracheal intubations, long-term tracheostomies, trauma, airway burns, and some systemic diseases. An effective and less invasive technique for both the early assessment and the early interventional treatment of acquired airway stenosis is therefore needed. Optical coherence tomography (OCT) has been proposed to have unique potential for early monitoring from the proliferative epithelium to the cartilage in acute airway injury. Additionally, stem cell therapy using adipose stem cells is being investigated as an option for early interventional treatment in airway and lung injury. Over the past decade, it has become possible to monitor the level of injury using OCT and to track the engraftment of stem cells using stem cell imaging in regenerative tissue. The purpose of this study was to assess the engraftment of exogenous adipose stem cells in injured tracheal epithelium with fluorescent microscopy and to detect and monitor the degree of airway injury in the same tracheal epithelium with OCT. OCT detected thickening of both the epithelium and basement membrane after tracheal scraping. The engraftment of adipose stem cells was successfully detected by fluorescent staining in the regenerative epithelium of injured tracheas. OCT has the potential to be a high-resolution imaging modality capable of detecting airway injury in combination with stem cell imaging in the same tracheal mucosa.

© 2013 Optical Society of America

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Optical Coherence Tomography

History
Original Manuscript: October 11, 2013
Revised Manuscript: December 7, 2013
Manuscript Accepted: December 12, 2013
Published: December 20, 2013

Citation
Yeh-Chan Ahn, Sung Won Kim, Sang Seok Hwang, Yu-Gyeong Chae, Andrew Sungwan Lee, Maan Hong Jung, Bong Kwon Chun, Sang Joon Lee, Eun-Kee Park, and Chulho Oak, "Optical imaging of subacute airway remodeling and adipose stem cell engraftment after airway injury," Biomed. Opt. Express 5, 312-321 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-1-312


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. D. J. Mathisen, “Surgery of the trachea,” Curr. Probl. Surg.35(6), 453–542 (1998). [PubMed]
  2. B. Minnigerode and H. G. Richter, “Pathophysiology of subglottic tracheal stenosis in childhood,” Prog. Pediatr. Surg.21, 1–7 (1987). [CrossRef] [PubMed]
  3. D. Preciado, R. T. Cotton, and M. J. Rutter, “Single-stage tracheal resection for severe tracheal stenosis in older children,” Int. J. Pediatr. Otorhinolaryngol.68(1), 1–6 (2004). [CrossRef] [PubMed]
  4. C. Personne, A. Colchen, M. Leroy, G. Vourc’h, and L. Toty, “Indications and technique for endoscopic laser resections in bronchology. A critical analysis based upon 2,284 resections,” J. Thorac. Cardiovasc. Surg.91(5), 710–715 (1986). [PubMed]
  5. A. R. Burningham, M. K. Wax, P. E. Andersen, E. C. Everts, and J. I. Cohen, “Metallic tracheal stents: complications associated with long-term use in the upper airway,” Ann. Otol. Rhinol. Laryngol.111(4), 285–290 (2002). [PubMed]
  6. M. Brenner, K. Kreuter, D. Mukai, T. Burney, S. Guo, J. Su, S. Mahon, A. Tran, L. Tseng, J. Ju, and Z. Chen, “Detection of acute smoke-induced airway injury in a New Zealand white rabbit model using optical coherence tomography,” J. Biomed. Opt.12(5), 051701 (2007). [CrossRef] [PubMed]
  7. J. L. Lin, A. Y. Yau, J. Boyd, A. Hamamoto, E. Su, L. Tracy, A. E. Heidari, A. H. Wang, G. Ahuja, Z. Chen, and B. J. Wong, “Real-time subglottic stenosis imaging using optical coherence tomography in the rabbit,” JAMA Otolaryngol. Head Neck Surg.139(5), 502–509 (2013). [CrossRef] [PubMed]
  8. S. Pulavendran, J. Vignesh, and C. Rose, “Differential anti-inflammatory and anti-fibrotic activity of transplanted mesenchymal vs. hematopoietic stem cells in carbon tetrachloride-induced liver injury in mice,” Int. Immunopharmacol.10(4), 513–519 (2010). [CrossRef] [PubMed]
  9. S. H. Lee, A. S. Jang, Y. E. Kim, J. Y. Cha, T. H. Kim, S. Jung, S. K. Park, Y. K. Lee, J. H. Won, Y. H. Kim, and C. S. Park, “Modulation of cytokine and nitric oxide by mesenchymal stem cell transfer in lung injury/fibrosis,” Respir. Res.11(1), 16 (2010). [CrossRef] [PubMed]
  10. D. W. Borthwick, M. Shahbazian, Q. T. Krantz, J. R. Dorin, and S. H. Randell, “Evidence for stem-cell niches in the tracheal epithelium,” Am. J. Respir. Cell Mol. Biol.24(6), 662–670 (2001). [CrossRef] [PubMed]
  11. Y. Nakagishi, Y. Morimoto, M. Fujita, Y. Ozeki, T. Maehara, and M. Kikuchi, “Rabbit model of airway stenosis induced by scraping of the tracheal mucosa,” Laryngoscope115(6), 1087–1092 (2005). [CrossRef] [PubMed]
  12. J. Xu, Y. Chen, Y. Yue, J. Sun, and L. Cui, “Reconstruction of epidural fat with engineered adipose tissue from adipose derived stem cells and PLGA in the rabbit dorsal laminectomy model,” Biomaterials33(29), 6965–6973 (2012). [CrossRef] [PubMed]
  13. K. A. Kreuter, S. B. Mahon, D. S. Mukai, J. Su, W. G. Jung, N. Narula, S. Guo, N. Wakida, C. Raub, M. W. Berns, S. C. George, Z. Chen, and M. Brenner, “Detection and monitoring of early airway injury effects of half-mustard (2-chloroethylethylsulfide) exposure using high-resolution optical coherence tomography,” J. Biomed. Opt.14(4), 044037 (2009). [CrossRef] [PubMed]
  14. S. W. Lee, A. E. Heidary, D. Yoon, D. Mukai, T. Ramalingam, S. Mahon, J. Yin, J. Jing, G. Liu, Z. Chen, and M. Brenner, “Quantification of airway thickness changes in smoke-inhalation injury using in-vivo 3-D endoscopic frequency-domain optical coherence tomography,” Biomed. Opt. Express2(2), 243–254 (2011). [CrossRef] [PubMed]
  15. M. Brenner, K. Kreuter, J. Ju, S. Mahon, L. Tseng, D. Mukai, T. Burney, S. Guo, J. Su, A. Tran, A. Batchinsky, L. C. Cancio, N. Narula, and Z. Chen, “In vivo optical coherence tomography detection of differences in regional large airway smoke inhalation induced injury in a rabbit model,” J. Biomed. Opt.13(3), 034001 (2008). [CrossRef] [PubMed]
  16. T. R. Weber, R. H. Connors, and T. F. Tracy., “Acquired tracheal stenosis in infants and children,” J. Thorac. Cardiovasc. Surg.102(1), 29–34 (1991). [PubMed]
  17. D. A. Chistiakov, “Endogenous and exogenous stem cells: a role in lung repair and use in airway tissue engineering and transplantation,” J. Biomed. Sci.17(1), 92–100 (2010). [CrossRef] [PubMed]
  18. F. Zhao, Y. F. Zhang, Y. G. Liu, J. J. Zhou, Z. K. Li, C. G. Wu, and H. W. Qi, “Therapeutic Effects of Bone Marrow-Derived Mesenchymal Stem Cells Engraftment on Bleomycin-Induced Lung Injury in Rats,” Transplant. Proc.40(5), 1700–1705 (2008). [CrossRef] [PubMed]
  19. A. E. Hegab, D. W. Nickerson, V. L. Ha, D. O. Darmawan, and B. N. Gomperts, “Repair and regeneration of tracheal surface epithelium and submucosal glands in a mouse model of hypoxic-ischemic injury,” Respirology17(7), 1101–1113 (2012). [CrossRef] [PubMed]
  20. G. Bhatia, V. Abraham, and L. Louis, “Tracheal granulation as a cause of unrecognized airway narrowing,” J. Anaesthesiol. Clin. Pharmacol.28(2), 235–238 (2012). [CrossRef] [PubMed]
  21. E. Puchelle, J. M. Zahm, J. M. Tournier, and C. Coraux, “Airway epithelial repair, regeneration, and remodeling after injury in chronic obstructive pulmonary disease,” Proc. Am. Thorac. Soc.3(8), 726–733 (2006). [CrossRef] [PubMed]
  22. D. L. Kraitchman, A. W. Heldman, E. Atalar, L. C. Amado, B. J. Martin, M. F. Pittenger, J. M. Hare, and J. W. M. Bulte, “In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction,” Circulation107(18), 2290–2293 (2003). [CrossRef] [PubMed]
  23. S. Y. Nam, L. M. Ricles, L. J. Suggs, and S. Y. Emelianov, “In vivo ultrasound and photoacoustic monitoring of mesenchymal stem cells labeled with gold nanotracers,” PLoS ONE7(5), e37267 (2012). [CrossRef] [PubMed]
  24. J. V. Jokerst, M. Thangaraj, P. J. Kempen, R. Sinclair, and S. S. Gambhir, “Photoacoustic imaging of mesenchymal stem cells in living mice via silica-coated gold nanorods,” ACS Nano6(7), 5920–5930 (2012). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited