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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 1 — Jan. 1, 2014
  • pp: 69–77

High-resolution visualization of mouse cardiac microvasculature using optical histology

Austin J. Moy, Patrick C. Lo, and Bernard Choi  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 1, pp. 69-77 (2014)
http://dx.doi.org/10.1364/BOE.5.000069


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Abstract

Cardiovascular disease typically is associated with dysfunction of the coronary vasculature and microvasculature. The study of cardiovascular disease typically involves imaging of the large coronary vessels and quantification of cardiac blood perfusion. These methods, however, are not well suited for imaging of the cardiac microvasculature. We used the optical histology method, which combines chemical optical clearing and optical imaging, to create high-resolution, wide-field maps of the cardiac microvasculature in ventral slices of mouse heart. We have demonstrated the ability of the optical histology method to enable wide-field visualization of the cardiac microvasculature in high-resolution and anticipate that optical histology may have significant impact in studying cardiovascular disease.

© 2013 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6930) Medical optics and biotechnology : Tissue

ToC Category:
Microscopy

History
Original Manuscript: August 2, 2013
Revised Manuscript: October 4, 2013
Manuscript Accepted: November 15, 2013
Published: December 4, 2013

Virtual Issues
Advances in Optics for Biotechnology, Medicine and Surgery (2013) Biomedical Optics Express

Citation
Austin J. Moy, Patrick C. Lo, and Bernard Choi, "High-resolution visualization of mouse cardiac microvasculature using optical histology," Biomed. Opt. Express 5, 69-77 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-1-69


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