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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 2 — Feb. 1, 2014
  • pp: 515–538

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Sergio Coda, Alex J. Thompson, Gordon T. Kennedy, Kim L. Roche, Lakshmana Ayaru, Devinder S. Bansi, Gordon W. Stamp, Andrew V. Thillainayagam, Paul M. W. French, and Chris Dunsby  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 2, pp. 515-538 (2014)
http://dx.doi.org/10.1364/BOE.5.000515


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Abstract

We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime −570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens.

© 2014 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.2680) Medical optics and biotechnology : Gastrointestinal
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(300.6500) Spectroscopy : Spectroscopy, time-resolved
(300.6540) Spectroscopy : Spectroscopy, ultraviolet
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:
Optics in Cancer Research

History
Original Manuscript: July 31, 2013
Revised Manuscript: October 11, 2013
Manuscript Accepted: November 26, 2013
Published: January 16, 2014

Virtual Issues
Optical Molecular Probes, Imaging, and Drug Delivery (2013) Biomedical Optics Express

Citation
Sergio Coda, Alex J. Thompson, Gordon T. Kennedy, Kim L. Roche, Lakshmana Ayaru, Devinder S. Bansi, Gordon W. Stamp, Andrew V. Thillainayagam, Paul M. W. French, and Chris Dunsby, "Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe," Biomed. Opt. Express 5, 515-538 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-2-515


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