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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 2 — Feb. 1, 2014
  • pp: 562–572

Non-invasive fluorescence imaging under ambient light conditions using a modulated ICCD and laser diode

Banghe Zhu, John C. Rasmussen, and Eva M. Sevick-Muraca  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 2, pp. 562-572 (2014)
http://dx.doi.org/10.1364/BOE.5.000562


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Abstract

One limitation of fluorescence molecular imaging that can limit clinical implementation and hamper small animal imaging is the inability to eliminate ambient light. Herein, we demonstrate the ability to conduct rapid non-invasive, far-red and near-infrared fluorescence imaging in living animals and a phantom under ambient light conditions using a modulated image intensified CCD (ICCD) and a laser diode operated in homodyne detection. By mapping AC amplitude from three planar images at varying phase delays, we show improvement in target-to-background ratios (TBR) and reasonable signal-to-noise ratios (SNR) over continuous wave measurements. The rapid approach can be used to accurately collect fluorescence in situations where ambient light cannot be spectrally conditioned or controlled, such as in the case of fluorescent molecular image-guided surgery.

© 2014 Optical Society of America

OCIS Codes
(040.1520) Detectors : CCD, charge-coupled device
(060.2630) Fiber optics and optical communications : Frequency modulation
(260.2510) Physical optics : Fluorescence

ToC Category:
Clinical Instrumentation

History
Original Manuscript: November 6, 2013
Revised Manuscript: January 22, 2014
Manuscript Accepted: January 22, 2014
Published: January 24, 2014

Citation
Banghe Zhu, John C. Rasmussen, and Eva M. Sevick-Muraca, "Non-invasive fluorescence imaging under ambient light conditions using a modulated ICCD and laser diode," Biomed. Opt. Express 5, 562-572 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-2-562


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