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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 3 — Mar. 1, 2014
  • pp: 763–777

Hadamard multiplexed fluorescence tomography

Ali Behrooz, Ali A. Eftekhar, and Ali Adibi  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 3, pp. 763-777 (2014)

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Depth-resolved three-dimensional (3D) reconstruction of fluorophore-tagged inclusions in fluorescence tomography (FT) poses a highly ill-conditioned problem as depth information must be extracted from boundary data. Due to the ill-posed nature of the FT inverse problem, noise and errors in the data can severely impair the accuracy of the 3D reconstructions. The signal-to-noise ratio (SNR) of the FT data strongly affects the quality of the reconstructions. Additionally, in FT scenarios where the fluorescent signal is weak, data acquisition requires lengthy integration times that result in excessive FT scan periods. Enhancing the SNR of FT data contributes to the robustness of the 3D reconstructions as well as the speed of FT scans. A major deciding factor in the SNR of the FT data is the power of the radiation illuminating the subject to excite the administered fluorescent reagents. In existing single-point illumination FT systems, the source power level is limited by the skin maximum radiation exposure levels. In this paper, we introduce and study the performance of a multiplexed fluorescence tomography system with orders-of-magnitude enhanced data SNR over existing systems. The proposed system allows for multi-point illumination of the subject without jeopardizing the information content of the FT measurements and results in highly robust reconstructions of fluorescent inclusions from noisy FT data. Improvements offered by the proposed system are validated by numerical and experimental studies.

© 2014 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6960) Medical optics and biotechnology : Tomography
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Image Reconstruction and Inverse Problems

Original Manuscript: October 21, 2013
Revised Manuscript: January 10, 2014
Manuscript Accepted: February 5, 2014
Published: February 18, 2014

Ali Behrooz, Ali A. Eftekhar, and Ali Adibi, "Hadamard multiplexed fluorescence tomography," Biomed. Opt. Express 5, 763-777 (2014)

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