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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 3 — Mar. 1, 2014
  • pp: 848–857

Detection of swelling of single isolated mitochondrion with optical microscopy

Daisuke Morikawa, Keita Kanematsu, Takahiro Shibata, Keisuke Haseda, Norihiro Umeda, and Yoshihiro Ohta  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 3, pp. 848-857 (2014)
http://dx.doi.org/10.1364/BOE.5.000848


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Abstract

Volume regulation under osmotic loading is one of the most fundamental functions in cells and organelles. However, the effective method to detect volume changes of a single organelle has not been developed. Here, we present a novel technique for detecting volume changes of a single isolated mitochondrion in aqueous solution based on the transmittance of the light through the mitochondrion. We found that 70% and 21% of mitochondria swelled upon addition of a hypotonic solution and Ca2+, respectively. These results show the potential of the present technique to detect the physiological volume changes of individual small organelles such as mitochondria.

© 2014 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Cell Studies

History
Original Manuscript: December 9, 2013
Revised Manuscript: January 13, 2014
Manuscript Accepted: February 14, 2014
Published: February 24, 2014

Citation
Daisuke Morikawa, Keita Kanematsu, Takahiro Shibata, Keisuke Haseda, Norihiro Umeda, and Yoshihiro Ohta, "Detection of swelling of single isolated mitochondrion with optical microscopy," Biomed. Opt. Express 5, 848-857 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-3-848


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