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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 3 — Mar. 1, 2014
  • pp: 921–931

Handheld multispectral fluorescence lifetime imaging system for in vivo applications

Shuna Cheng, Rodrigo M. Cuenca, Boang Liu, Bilal H. Malik, Joey M. Jabbour, Kristen C. Maitland, John Wright, Yi-Shing Lisa Cheng, and Javier A. Jo  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 3, pp. 921-931 (2014)

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There is an increasing interest in the application of fluorescence lifetime imaging (FLIM) for medical diagnosis. Central to the clinical translation of FLIM technology is the development of compact and high-speed clinically compatible systems. We present a handheld probe design consisting of a small maneuverable box fitted with a rigid endoscope, capable of continuous lifetime imaging at multiple emission bands simultaneously. The system was characterized using standard fluorescent dyes. The performance was then further demonstrated by imaging a hamster cheek pouch in vivo, and oral mucosa tissue both ex vivo and in vivo, all using safe and permissible exposure levels. Such a design can greatly facilitate the evaluation of FLIM for oral cancer imaging in vivo.

© 2014 Optical Society of America

OCIS Codes
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:
Clinical Instrumentation

Original Manuscript: January 10, 2014
Revised Manuscript: February 19, 2014
Manuscript Accepted: February 19, 2014
Published: February 26, 2014

Shuna Cheng, Rodrigo M. Cuenca, Boang Liu, Bilal H. Malik, Joey M. Jabbour, Kristen C. Maitland, John Wright, Yi-Shing Lisa Cheng, and Javier A. Jo, "Handheld multispectral fluorescence lifetime imaging system for in vivo applications," Biomed. Opt. Express 5, 921-931 (2014)

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