OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 3 — Mar. 1, 2014
  • pp: 921–931

Handheld multispectral fluorescence lifetime imaging system for in vivo applications

Shuna Cheng, Rodrigo M. Cuenca, Boang Liu, Bilal H. Malik, Joey M. Jabbour, Kristen C. Maitland, John Wright, Yi-Shing Lisa Cheng, and Javier A. Jo  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 3, pp. 921-931 (2014)
http://dx.doi.org/10.1364/BOE.5.000921


View Full Text Article

Enhanced HTML    Acrobat PDF (3807 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

There is an increasing interest in the application of fluorescence lifetime imaging (FLIM) for medical diagnosis. Central to the clinical translation of FLIM technology is the development of compact and high-speed clinically compatible systems. We present a handheld probe design consisting of a small maneuverable box fitted with a rigid endoscope, capable of continuous lifetime imaging at multiple emission bands simultaneously. The system was characterized using standard fluorescent dyes. The performance was then further demonstrated by imaging a hamster cheek pouch in vivo, and oral mucosa tissue both ex vivo and in vivo, all using safe and permissible exposure levels. Such a design can greatly facilitate the evaluation of FLIM for oral cancer imaging in vivo.

© 2014 Optical Society of America

OCIS Codes
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:
Clinical Instrumentation

History
Original Manuscript: January 10, 2014
Revised Manuscript: February 19, 2014
Manuscript Accepted: February 19, 2014
Published: February 26, 2014

Citation
Shuna Cheng, Rodrigo M. Cuenca, Boang Liu, Bilal H. Malik, Joey M. Jabbour, Kristen C. Maitland, John Wright, Yi-Shing Lisa Cheng, and Javier A. Jo, "Handheld multispectral fluorescence lifetime imaging system for in vivo applications," Biomed. Opt. Express 5, 921-931 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-3-921


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Springer, New York, 2006).
  2. J. Mizeret, T. Stepinac, M. Hansroul, A. Studzinski, H. van den Bergh, and G. Wagnieres, “Instrumentation for real-time fluorescence lifetime imaging in endoscopy,” Rev. Sci. Instrum.70(12), 4689–4701 (1999). [CrossRef]
  3. I. Munro, J. McGinty, N. Galletly, J. Requejo-Isidro, P. M. P. Lanigan, D. S. Elson, C. Dunsby, M. A. A. Neil, M. J. Lever, G. W. H. Stamp, and P. M. W. French, “Toward the clinical application of time-domain fluorescence lifetime imaging,” J. Biomed. Opt.10(5), 051403 (2005). [CrossRef] [PubMed]
  4. J. Requejo-Isidro, J. McGinty, I. Munro, D. S. Elson, N. P. Galletly, M. J. Lever, M. A. A. Neil, G. W. H. Stamp, P. M. W. French, P. A. Kellett, J. D. Hares, and A. K. L. Dymoke-Bradshaw, “High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging,” Opt. Lett.29(19), 2249–2251 (2004). [CrossRef] [PubMed]
  5. G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J. Biophotonics3(1-2), 103–107 (2010). [CrossRef] [PubMed]
  6. G. O. Fruhwirth, S. Ameer-Beg, R. Cook, T. Watson, T. Ng, and F. Festy, “Fluorescence lifetime endoscopy using TCSPC for the measurement of FRET in live cells,” Opt. Express18(11), 11148–11158 (2010). [CrossRef] [PubMed]
  7. G. T. Kennedy, S. Coda, A. J. Thompson, D. S. Elson, M. A. A. Neil, G. W. Stamp, A. Thillainayagam, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “Fluorescence lifetime imaging endoscopy,” Proc. SPIE7893, 789308 (2011). [CrossRef]
  8. D. S. Elson, J. A. Jo, and L. Marcu, “Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimens,” New J. Phys.9(5), 127 (2007). [CrossRef] [PubMed]
  9. S. Cheng, J. J. Rico-Jimenez, J. Jabbour, B. Malik, K. C. Maitland, J. Wright, Y. S. Cheng, and J. A. Jo, “Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging,” Opt. Lett.38(9), 1515–1517 (2013). [CrossRef] [PubMed]
  10. H. Xie, J. Bec, J. Liu, Y. Sun, M. Lam, D. R. Yankelevich, and L. Marcu, “Multispectral scanning time-resolved fluorescence spectroscopy (TRFS) technique for intravascular diagnosis,” Biomed. Opt. Express3(7), 1521–1533 (2012). [CrossRef] [PubMed]
  11. D. G. Ouzounov, D. R. Rivera, W. W. Webb, J. Bentley, and C. Xu, “Miniature varifocal objective lens for endomicroscopy,” Opt. Lett.38(16), 3103–3106 (2013). [CrossRef] [PubMed]
  12. S. Shrestha, B. E. Applegate, J. Park, X. Xiao, P. Pande, and J. A. Jo, “High-speed multispectral fluorescence lifetime imaging implementation for in vivo applications,” Opt. Lett.35(15), 2558–2560 (2010). [CrossRef] [PubMed]
  13. P. Pande and J. A. Jo, “Automated analysis of fluorescence lifetime imaging microscopy (FLIM) data based on the Laguerre deconvolution method,” IEEE Trans. Biomed. Eng.58(1), 172–181 (2011). [CrossRef] [PubMed]
  14. Y. Sun, H. Xie, J. Liu, M. Lam, A. J. Chaudhari, F. Zhou, J. Bec, D. R. Yankelevich, A. Dobbie, S. L. Tinling, R. F. Gandour-Edwards, W. L. Monsky, D. G. Farwell, and L. Marcu, “In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma,” J. Biomed. Opt.17(11), 116003 (2012). [CrossRef] [PubMed]
  15. American National Standards Institute, Safe Use of Lasers, ANSI Z136.1–2007, (Laser Institute of America, 2007).
  16. M. Yuvaraj, K. Udayakumar, V. Jayanth, A. Prakasa Rao, G. Bharanidharan, D. Koteeswaran, B. D. Munusamy, C. Murali Krishna, and S. Ganesan, “Fluorescence spectroscopic characterization of salivary metabolites of oral cancer patients,” J. Photochem. Photobiol. B130C, 153–160 (2013). [PubMed]
  17. H. M. Chen, C. P. Chiang, C. You, T. C. Hsiao, and C. Y. Wang, “Time-resolved autofluorescence spectroscopy for classifying normal and premalignant oral tissues,” Lasers Surg. Med.37(1), 37–45 (2005). [CrossRef] [PubMed]
  18. Z. Nie, R. An, J. E. Hayward, T. J. Farrell, and Q. Fang, “Hyperspectral fluorescence lifetime imaging for optical biopsy,” J. Biomed. Opt.18(9), 096001 (2013). [CrossRef] [PubMed]
  19. J. Bec, D. M. Ma, D. R. Yankelevich, J. Liu, W. T. Ferrier, J. Southard, and L. Marcu, “Multispectral fluorescence lifetime imaging system for intravascular diagnostics with ultrasound guidance: in vivo validation in swine arteries,” J. Biophotonics doi: (2013). [CrossRef]
  20. Y. Sun, J. E. Phipps, J. Meier, N. Hatami, B. Poirier, D. S. Elson, D. G. Farwell, and L. Marcu, “Endoscopic Fluorescence Lifetime Imaging for In Vivo Intraoperative Diagnosis of Oral Carcinoma,” Microsc. Microanal.19(4), 791–798 (2013). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4 Fig. 5
 

Supplementary Material


» Media 1: AVI (396 KB)     
» Media 2: AVI (1370 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited