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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 3 — Mar. 1, 2014
  • pp: 961–974

In vivo adaptive optics microvascular imaging in diabetic patients without clinically severe diabetic retinopathy

Stephen A. Burns, Ann E. Elsner, Toco Y. Chui, Dean A. VanNasdale, Jr., Christopher A. Clark, Thomas J Gast, Victor E. Malinovsky, and Anh-Danh T. Phan  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 3, pp. 961-974 (2014)
http://dx.doi.org/10.1364/BOE.5.000961


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Abstract

We used a confocal adaptive optics scanning laser ophthalmoscope (AOSLO) to image the retina of subjects with non-proliferative diabetic retinopathy (NPDR). To improve visualization of different retinal features, the size and alignment of the confocal aperture were varied. The inner retinal layers contained clearly visualized retinal vessels. In diabetic subjects there was extensive capillary remodeling despite the subjects having only mild or moderate NPDR. Details of the retinal microvasculature were readily imaged with a larger confocal aperture. Hard exudates were observed with the AOSLO in all imaging modes. Photoreceptor layer images showed regions of bright cones and dark areas, corresponding in location to overlying vascular abnormalities and retinal edema. Clinically undetected intraretinal vessel remodeling and varying blood flow patterns were found. Perifoveal capillary diameters were larger in the diabetic subjects (p<0.01), and small arteriolar walls were thickened, based on wall to lumen measurements (p<.05). The results suggest that existing clinical classifications based on lower magnification clinical assessment may not adequately measure key vascular differences among individuals with NPDR.

© 2014 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4460) Medical optics and biotechnology : Ophthalmic optics and devices
(170.4470) Medical optics and biotechnology : Ophthalmology

ToC Category:
Ophthalmology Applications

History
Original Manuscript: January 15, 2014
Revised Manuscript: January 15, 2014
Manuscript Accepted: February 21, 2014
Published: February 27, 2014

Citation
Stephen A. Burns, Ann E. Elsner, Toco Y. Chui, Dean A. VanNasdale, Christopher A. Clark, Thomas J Gast, Victor E. Malinovsky, and Anh-Danh T. Phan, "In vivo adaptive optics microvascular imaging in diabetic patients without clinically severe diabetic retinopathy," Biomed. Opt. Express 5, 961-974 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-3-961


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