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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 4 — Apr. 1, 2014
  • pp: 1000–1013

Optical stimulation enables paced electrophysiological studies in embryonic hearts

Yves T. Wang, Shi Gu, Pei Ma, Michiko Watanabe, Andrew M. Rollins, and Michael W. Jenkins  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 4, pp. 1000-1013 (2014)

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Cardiac electrophysiology plays a critical role in the development and function of the heart. Studies of early embryonic electrical activity have lacked a viable point stimulation technique to pace in vitro samples. Here, optical pacing by high-precision infrared stimulation is used to pace excised embryonic hearts, allowing electrophysiological parameters to be quantified during pacing at varying rates with optical mapping. Combined optical pacing and optical mapping enables electrophysiological studies in embryos under more physiological conditions and at varying heart rates, allowing detection of abnormal conduction and comparisons between normal and pathological electrical activity during development in various models.

© 2014 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(140.3460) Lasers and laser optics : Lasers
(140.6810) Lasers and laser optics : Thermal effects
(170.2520) Medical optics and biotechnology : Fluorescence microscopy

ToC Category:
Optogenetics and Optical Stimulation

Original Manuscript: December 17, 2013
Revised Manuscript: February 13, 2014
Manuscript Accepted: February 21, 2014
Published: February 28, 2014

Yves T. Wang, Shi Gu, Pei Ma, Michiko Watanabe, Andrew M. Rollins, and Michael W. Jenkins, "Optical stimulation enables paced electrophysiological studies in embryonic hearts," Biomed. Opt. Express 5, 1000-1013 (2014)

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