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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 4 — Apr. 1, 2014
  • pp: 1014–1025

Nanosecond laser pulse stimulation of spiral ganglion neurons and model cells

Alexander Rettenmaier, Thomas Lenarz, and Günter Reuter  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 4, pp. 1014-1025 (2014)
http://dx.doi.org/10.1364/BOE.5.001014


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Abstract

Optical stimulation of the inner ear has recently attracted attention, suggesting a higher frequency resolution compared to electrical cochlear implants due to its high spatial stimulation selectivity. Although the feasibility of the effect is shown in multiple in vivo experiments, the stimulation mechanism remains open to discussion. Here we investigate in single-cell measurements the reaction of spiral ganglion neurons and model cells to irradiation with a nanosecond-pulsed laser beam over a broad wavelength range from 420 nm up to 1950 nm using the patch clamp technique. Cell reactions were wavelength- and pulse-energy-dependent but too small to elicit action potentials in the investigated spiral ganglion neurons. As the applied radiant exposure was much higher than the reported threshold for in vivo experiments in the same laser regime, we conclude that in a stimulation paradigm with nanosecond-pulses, direct neuronal stimulation is not the main cause of optical cochlea stimulation.

© 2014 Optical Society of America

OCIS Codes
(140.3600) Lasers and laser optics : Lasers, tunable
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4940) Medical optics and biotechnology : Otolaryngology
(350.5340) Other areas of optics : Photothermal effects
(170.1065) Medical optics and biotechnology : Acousto-optics
(110.5125) Imaging systems : Photoacoustics

ToC Category:
Optogenetics and Optical Stimulation

History
Original Manuscript: November 6, 2013
Revised Manuscript: December 26, 2013
Manuscript Accepted: January 13, 2014
Published: March 3, 2014

Citation
Alexander Rettenmaier, Thomas Lenarz, and Günter Reuter, "Nanosecond laser pulse stimulation of spiral ganglion neurons and model cells," Biomed. Opt. Express 5, 1014-1025 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-4-1014


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