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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 4 — Apr. 1, 2014
  • pp: 1038–1049

Effect of temperature and fixation on the optical properties of atherosclerotic tissue: a validation study of an ex-vivo whole heart cadaveric model

Muthukaruppan Gnanadesigan, Gijs van Soest, Stephen White, Simon Scoltock, Giovanni J Ughi, Andreas Baumbach, Antonius FW van der Steen, Evelyn Regar, and Thomas W Johnson  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 4, pp. 1038-1049 (2014)

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Atherosclerotic plaque composition can be imaged using the optical attenuation coefficient derived from intravascular optical coherence tomography (OCT) data. The relation between optical properties and tissue type has been established on autopsy tissues. In this study, we validate an ex-vivo model for the effect of temperature and tissue fixation on optical parameters. We studied the optical attenuation of human coronary arteries at three temperatures, before and after formalin fixation. We developed an en-face longitudinal display of attenuation data of the OCT pullbacks. Using the unfixed, body-temperature condition image as a standard, and after extensive registration with other condition images, we quantify the differences in optical attenuation and the backscattered intensity. The results suggest that tissue fixation and temperature do not introduce systematic errors in studies of arterial optical properties.

© 2014 Optical Society of America

OCIS Codes
(110.2960) Imaging systems : Image analysis
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Cardiovascular Applications

Original Manuscript: December 5, 2013
Revised Manuscript: January 24, 2014
Manuscript Accepted: February 1, 2014
Published: March 3, 2014

Muthukaruppan Gnanadesigan, Gijs van Soest, Stephen White, Simon Scoltock, Giovanni J Ughi, Andreas Baumbach, Antonius FW van der Steen, Evelyn Regar, and Thomas W Johnson, "Effect of temperature and fixation on the optical properties of atherosclerotic tissue: a validation study of an ex-vivo whole heart cadaveric model," Biomed. Opt. Express 5, 1038-1049 (2014)

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