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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 4 — Apr. 1, 2014
  • pp: 1290–1295

Screening hypochromism (sieve effect) in red blood cells: a quantitative analysis

K. Razi Naqvi  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 4, pp. 1290-1295 (2014)

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Multiwavelength UV-visible spectroscopy, Kramers-Kronig analysis, and several other experimental and theoretical tools have been applied over the last several decades to fathom absorption and scattering of light by suspensions of micron-sized pigmented particles, including red blood cells, but a satisfactory quantitative analysis of the difference between the absorption spectra of suspension of intact and lysed red blood cells is still lacking. It is stressed that such a comparison is meaningful only if the pertinent spectra are free from, or have been corrected for, scattering losses, and it is shown that Duysens’ theory can, whereas that of Vekshin cannot, account satisfactorily for the observed hypochromism of suspensions of red blood cells.

© 2014 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring

ToC Category:
Cell Studies

Original Manuscript: February 3, 2014
Revised Manuscript: March 20, 2014
Manuscript Accepted: March 23, 2014
Published: March 28, 2014

K. Razi Naqvi, "Screening hypochromism (sieve effect) in red blood cells: a quantitative analysis," Biomed. Opt. Express 5, 1290-1295 (2014)

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