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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 5 — May. 1, 2014
  • pp: 1321–1335

Three-dimensional transillumination image reconstruction for small animal with new scattering suppression technique

Trung Nghia Tran, Kohei Yamamoto, Takeshi Namita, Yuji Kato, and Koichi Shimizu  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 5, pp. 1321-1335 (2014)

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To realize three-dimensional (3D) optical imaging of the internal structure of an animal body, we have developed a new technique to reconstruct optical computed tomography (optical CT) images from two-dimensional (2D) transillumination images. In transillumination imaging of an animal body using near-infrared light, the image is blurred because of the strong scattering in the tissue. To overcome this problem, we propose a novel technique to apply the point spread function (PSF) for a light source located inside the medium to the transilluminated image of light-absorbing structure. The problem of the depth-dependence of PSF was solved in the calculation of the projection image in the filtered back-projection method. The effectiveness of the proposed technique was assessed in the experiments with a model phantom and a mouse. These analyses verified the feasibility of the practical 3D imaging of the internal light-absorbing structure of a small animal.

© 2014 Optical Society of America

OCIS Codes
(100.1830) Image processing : Deconvolution
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6960) Medical optics and biotechnology : Tomography
(170.7050) Medical optics and biotechnology : Turbid media

ToC Category:
Image Reconstruction and Inverse Problems

Original Manuscript: January 23, 2014
Revised Manuscript: March 6, 2014
Manuscript Accepted: March 7, 2014
Published: April 1, 2014

Trung Nghia Tran, Kohei Yamamoto, Takeshi Namita, Yuji Kato, and Koichi Shimizu, "Three-dimensional transillumination image reconstruction for small animal with new scattering suppression technique," Biomed. Opt. Express 5, 1321-1335 (2014)

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  1. R. Bright, Diseases of the brain and nervous system vol. II (London: Longman, 1831).
  2. T. B. Curling, A practical treatise on the diseases of the testis and of the spermatic cord and scrotum (London: Samuel Highley, 1843).
  3. M. Cutler, “Transillumination as an aid in the diagnosis of breast lesions,” Surg. Gynecol. Obstet.48, 721–728 (1929).
  4. J. C. Hebden, S. R. Arridge, and D. T. Delpy, “Optical imaging in medicine: I. Experimental techniques,” Phys. Med. Biol.42(5), 825–840 (1997). [CrossRef] [PubMed]
  5. R. Weissleder and V. Ntziachristos, “Shedding light onto live molecular targets,” Nat. Med.9(1), 123–128 (2003). [CrossRef] [PubMed]
  6. A. H. Hielscher, “Optical tomographic imaging of small animals,” Curr. Opin. Biotechnol.16(1), 79–88 (2005). [CrossRef] [PubMed]
  7. A. P. Gibson, J. C. Hebden, and S. R. Arridge, “Recent advances in diffuse optical imaging,” Phys. Med. Biol.50(4), R1–R43 (2005). [CrossRef] [PubMed]
  8. V. Ntziachristos, “Fluorescence molecular imaging,” Annu. Rev. Biomed. Eng.8(1), 1–33 (2006). [CrossRef] [PubMed]
  9. A. Gibson and H. Dehghani, “Diffuse optical imaging,” Philos Trans A Math Phys Eng Sci367(1900), 3055–3072 (2009). [CrossRef] [PubMed]
  10. F. Leblond, S. C. Davis, P. A. Valdés, and B. W. Pogue, “Pre-clinical whole-body fluorescence imaging: Review of instruments, methods and applications,” J. Photochem. Photobiol. B98(1), 77–94 (2010). [CrossRef] [PubMed]
  11. B. D’Alessandro and A. P. Dhawan, “Transillumination imaging for blood oxygen saturation estimation of skin lesions,” IEEE Trans. Biomed. Eng.59(9), 2660–2667 (2012). [CrossRef] [PubMed]
  12. J. A. Guggenheim, H. R. A. Basevi, J. Frampton, I. B. Styles, and H. Dehghani, “Multi-modal molecular diffuse optical tomography system for small animal imaging,” Meas. Sci. Technol.24(10), 105405 (2013). [CrossRef]
  13. F. F. Jöbsis, “Noninvasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters,” Science198(4323), 1264–1267 (1977). [CrossRef] [PubMed]
  14. M. Cope and D. T. Delpy, “System for long-term measurement of cerebral blood and tissue oxygenation on newborn infants by near infra-red transillumination,” Med. Biol. Eng. Comput.26(3), 289–294 (1988). [CrossRef] [PubMed]
  15. G. Gratton, P. M. Corballis, E. Cho, M. Fabiani, and D. C. Hood, “Shades of gray matter: noninvasive optical images of human brain responses during visual stimulation,” Psychophysiology32(5), 505–509 (1995). [CrossRef] [PubMed]
  16. E. Okada, M. Firbank, M. Schweiger, S. R. Arridge, M. Cope, and D. T. Delpy, “Theoretical and experimental investigation of near-infrared light propagation in a model of the adult head,” Appl. Opt.36(1), 21–31 (1997). [CrossRef] [PubMed]
  17. F. F. Jobsis-Vandervliet, “Discovery of the near-infrared window into the body and the early development of near-infrared spectroscopy,” J. Biomed. Opt.4(4), 392–396 (1999). [CrossRef] [PubMed]
  18. Y. Taka, Y. Kato, and K. Shimizu, “Transillumination imaging of physiological functions by NIR light,” in Proceedings of IEEE Conference on 22nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (IEEE, 2000), pp. 771–774. [CrossRef]
  19. H. Obrig and A. Villringer, “Beyond the visible--imaging the human brain with light,” J. Cereb. Blood Flow Metab.23(1), 1–18 (2003). [CrossRef] [PubMed]
  20. Y. Hoshi, “Functional near-infrared optical imaging: utility and limitations in human brain mapping,” Psychophysiology40(4), 511–520 (2003). [CrossRef] [PubMed]
  21. I. Nishidate, Y. Aizu, and H. Mishina, “Depth visualization of a local blood region in skin tissue by use of diffuse reflectance images,” Opt. Lett.30(16), 2128–2130 (2005). [CrossRef] [PubMed]
  22. M. Wolf, M. Ferrari, and V. Quaresima, “Progress of near-infrared spectroscopy and topography for brain and muscle clinical applications,” J. Biomed. Opt.12(6), 062104 (2007). [CrossRef] [PubMed]
  23. T. Hamaoka, K. K. McCully, V. Quaresima, K. Yamamoto, and B. Chance, “Near-infrared spectroscopy/imaging for monitoring muscle oxygenation and oxidative metabolism in healthy and diseased humans,” J. Biomed. Opt.12(6), 062105 (2007). [CrossRef] [PubMed]
  24. A. Y. Bluestone, G. Abdoulaev, C. Schmitz, R. L. Barbour, and A. H. Hielscher, “Three-dimensional optical tomography of hemodynamics in the human head,” Opt. Express9(6), 272–286 (2001). [CrossRef] [PubMed]
  25. D. A. Boas, D. H. Brooks, E. L. Miller, C. A. DiMarzio, M. Kilmer, R. J. Gaudette, and Q. Zhang, “Imaging the body with diffuse optical tomography,” IEEE Signal Process. Mag.18(6), 57–75 (2001). [CrossRef]
  26. H. Jiang, Y. Xu, N. Iftimia, J. Eggert, K. Klove, L. Baron, and L. Fajardo, “Three-dimensional optical tomographic imaging of breast in a human subject,” IEEE Trans. Med. Imaging20(12), 1334–1340 (2001). [CrossRef] [PubMed]
  27. A. H. Hielscher, A. Y. Bluestone, G. S. Abdoulaev, A. D. Klose, J. Lasker, M. Stewart, U. Netz, and J. Beuthan, “Near-infrared diffuse optical tomography,” Dis. Markers18(5-6), 313–337 (2002). [CrossRef] [PubMed]
  28. F. Gao, H. Zhao, and Y. Yamada, “Improvement of image quality in diffuse optical tomography by use of full time-resolved data,” Appl. Opt.41(4), 778–791 (2002). [CrossRef] [PubMed]
  29. J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, “Three-dimensional optical tomography of the premature infant brain,” Phys. Med. Biol.47(23), 4155–4166 (2002). [CrossRef] [PubMed]
  30. H. Dehghani, B. W. Pogue, S. P. Poplack, and K. D. Paulsen, “Multiwavelength three-dimensional near-infrared tomography of the breast: initial simulation, phantom, and clinical results,” Appl. Opt.42(1), 135–145 (2003). [CrossRef] [PubMed]
  31. J. P. Culver, A. M. Siegel, J. J. Stott, and D. A. Boas, “Volumetric diffuse optical tomography of brain activity,” Opt. Lett.28(21), 2061–2063 (2003). [CrossRef] [PubMed]
  32. D. A. Boas, K. Chen, D. Grebert, and M. A. Franceschini, “Improving the diffuse optical imaging spatial resolution of the cerebral hemodynamic response to brain activation in humans,” Opt. Lett.29(13), 1506–1508 (2004). [CrossRef] [PubMed]
  33. B. W. Pogue, S. C. Davis, X. Song, B. A. Brooksby, H. Dehghani, and K. D. Paulsen, “Image analysis methods for diffuse optical tomography,” J. Biomed. Opt.11(3), 033001 (2006). [CrossRef] [PubMed]
  34. L. C. Enfield, A. P. Gibson, N. L. Everdell, D. T. Delpy, M. Schweiger, S. R. Arridge, C. Richardson, M. Keshtgar, M. Douek, and J. C. Hebden, “Three-dimensional time-resolved optical mammography of the uncompressed breast,” Appl. Opt.46(17), 3628–3638 (2007). [CrossRef] [PubMed]
  35. K. Shimizu, K. Tochio, and Y. Kato, “Improvement of transcutaneous fluorescent images with a depth-dependent point-spread function,” Appl. Opt.44(11), 2154–2161 (2005). [CrossRef] [PubMed]
  36. D. S. C. Biggs and M. Andrews, “Acceleration of iterative image restoration algorithms,” Appl. Opt.36(8), 1766–1775 (1997). [CrossRef] [PubMed]
  37. R. J. Hanisch, R. L. White, and R. L. Gilliland, “Deconvolution of hubble space telescope images and spectra,” in Deconvolution of Images and Spectra, P.A. Jansson, ed. (Academic Press, Boston, MA, 1997).
  38. Science Council of Japan, Guidelines for proper conduct of animal experiments (2006).

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