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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 5 — May. 1, 2014
  • pp: 1355–1362

Photo-induced processes in collagen-hypericin system revealed by fluorescence spectroscopy and multiphoton microscopy

V. Hovhannisyan, H. W. Guo, A. Hovhannisyan, V. Ghukasyan, T. Buryakina, Y. F. Chen, and C. Y. Dong  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 5, pp. 1355-1362 (2014)

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Collagen is the main structural protein and the key determinant of mechanical and functional properties of tissues and organs. Proper balance between synthesis and degradation of collagen molecules is critical for maintaining normal physiological functions. In addition, collagen influences tumor development and drug delivery, which makes it a potential cancer therapy target. Using second harmonic generation, two-photon excited fluorescence microscopy, and spectrofluorimetry, we show that the natural pigment hypericin induces photosensitized destruction of collagen-based tissues. We demonstrate that hypericin–mediated processes in collagen fibers are irreversible and may be used for the treatment of cancer and collagen-related disorders.

© 2014 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Optical Therapies and Photomodificaton

Original Manuscript: December 24, 2013
Revised Manuscript: January 20, 2014
Manuscript Accepted: January 20, 2014
Published: April 2, 2014

V. Hovhannisyan, H. W. Guo, A. Hovhannisyan, V. Ghukasyan, T. Buryakina, Y. F. Chen, and C. Y. Dong, "Photo-induced processes in collagen-hypericin system revealed by fluorescence spectroscopy and multiphoton microscopy," Biomed. Opt. Express 5, 1355-1362 (2014)

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