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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 5 — May. 1, 2014
  • pp: 1554–1568

Fully automated digital holographic processing for monitoring the dynamics of a vesicle suspension under shear flow

Christophe Minetti, Thomas Podgorski, Gwennou Coupier, and Frank Dubois  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 5, pp. 1554-1568 (2014)

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We investigate the dynamics of a vesicle suspension under shear flow between plates using DHM with a spatially reduced coherent source. Holograms are grabbed at a frequency of 24 frames/sec. The distribution of the vesicle suspension is obtained after numerical processing of the digital holograms sequence resulting in a 4D distribution. Obtaining this distribution is not straightforward and requires special processing to automate the analysis. We present an original method that fully automates the analysis and provides distributions that are further analyzed to extract physical properties of the fluid. Details of the numerical implementation, as well as sample experimental results are presented.

© 2014 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(100.0100) Image processing : Image processing
(100.2000) Image processing : Digital image processing
(100.6890) Image processing : Three-dimensional image processing
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(090.1995) Holography : Digital holography

ToC Category:
Image Processing

Original Manuscript: February 18, 2014
Revised Manuscript: April 10, 2014
Manuscript Accepted: April 11, 2014
Published: April 17, 2014

Christophe Minetti, Thomas Podgorski, Gwennou Coupier, and Frank Dubois, "Fully automated digital holographic processing for monitoring the dynamics of a vesicle suspension under shear flow," Biomed. Opt. Express 5, 1554-1568 (2014)

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