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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 5 — May. 1, 2014
  • pp: 1588–1609

Optimal lens design and use in laser-scanning microscopy

Adrian Negrean and Huibert D. Mansvelder  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 5, pp. 1588-1609 (2014)
http://dx.doi.org/10.1364/BOE.5.001588


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Abstract

In laser-scanning microscopy often an off-the-shelf achromatic doublet is used as a scan lens which can reduce the available diffraction-limited field-of-view (FOV) by a factor of 3 and introduce chromatic aberrations that are scan angle dependent. Here we present several simple lens designs of superior quality that fully make use of high-NA low-magnification objectives, offering diffraction-limited imaging over a large FOV and wavelength range. We constructed a two-photon laser-scanning microscope with optimized custom lenses which had a near diffraction limit point-spread-function (PSF) with less than 3.6% variation over a 400 µm FOV and less than 0.5 µm lateral color between 750 and 1050 nm.

© 2014 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.1790) Microscopy : Confocal microscopy
(220.3620) Optical design and fabrication : Lens system design
(220.3630) Optical design and fabrication : Lenses
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: January 17, 2014
Revised Manuscript: March 23, 2014
Manuscript Accepted: March 23, 2014
Published: April 18, 2014

Citation
Adrian Negrean and Huibert D. Mansvelder, "Optimal lens design and use in laser-scanning microscopy," Biomed. Opt. Express 5, 1588-1609 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-5-1588


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