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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 6 — Jun. 1, 2014
  • pp: 1886–1894

Double nanohole optical tweezers visualize protein p53 suppressing unzipping of single DNA-hairpins

Abhay Kotnala and Reuven Gordon  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 6, pp. 1886-1894 (2014)

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Here we report on the use of double-nanohole (DNH) optical tweezers as a label-free and free-solution single-molecule probe for protein–DNA interactions. Using this approach, we demonstrate the unzipping of individual 10 base pair DNA-hairpins, and quantify how tumor suppressor p53 protein delays the unzipping. From the Arrhenius behavior, we find the energy barrier to unzipping introduced by p53 to be 2 × 10−20 J, whereas cys135ser mutant p53 does not show suppression of unzipping, which gives clues to its functional inability to suppress tumor growth. This transformative approach to single molecule analysis allows for ultra-sensitive detection and quantification of protein–DNA interactions to revolutionize the fight against genetic diseases.

© 2014 Optical Society of America

OCIS Codes
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(350.4855) Other areas of optics : Optical tweezers or optical manipulation
(310.6628) Thin films : Subwavelength structures, nanostructures

ToC Category:
Optical Traps, Manipulation, and Tracking

Original Manuscript: March 13, 2014
Revised Manuscript: May 17, 2014
Manuscript Accepted: May 19, 2014
Published: May 21, 2014

Abhay Kotnala and Reuven Gordon, "Double nanohole optical tweezers visualize protein p53 suppressing unzipping of single DNA-hairpins," Biomed. Opt. Express 5, 1886-1894 (2014)

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