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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 6 — Jun. 1, 2014
  • pp: 1895–1912

Enhancing the isotropy of lateral resolution in coherent structured illumination microscopy

Joo Hyun Park, Jae Yong Lee, and Eun Seong Lee  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 6, pp. 1895-1912 (2014)

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We present a method to improve the isotropy of spatial resolution in a structured illumination microscopy (SIM) implemented for imaging non-fluorescent samples. To alleviate the problem of anisotropic resolution involved with the previous scheme of coherent SIM that employs the two orthogonal standing-wave illumination, referred to as the orthogonal SIM, we introduce a hexagonal-lattice illumination that incorporates three standing-wave fields simultaneously superimposed at the orientations equally divided in the lateral plane. A theoretical formulation is worked out rigorously for the coherent image formation with such a simultaneous multiple-beam illumination and an explicit Fourier-domain framework is derived for reconstructing an image with enhanced resolution. Using a computer-synthesized resolution target as a 2D coherent sample, we perform numerical simulations to examine the imaging characteristics of our three-angle SIM compared with the orthogonal SIM. The investigation on the 2D resolving power with the various test patterns of different periods and orientations reveal that the orientation-dependent undulation of lateral resolution can be reduced from 27% to 8% by using the three-angle SIM while the best resolution (0.54 times the resolution limit of conventional coherent imaging) in the directions of structured illumination is slightly deteriorated by 4.6% from that of the orthogonal SIM.

© 2014 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.0180) Imaging systems : Microscopy
(110.1650) Imaging systems : Coherence imaging
(110.2990) Imaging systems : Image formation theory
(110.3010) Imaging systems : Image reconstruction techniques

ToC Category:

Original Manuscript: April 11, 2014
Revised Manuscript: May 12, 2014
Manuscript Accepted: May 19, 2014
Published: May 21, 2014

Joo Hyun Park, Jae Yong Lee, and Eun Seong Lee, "Enhancing the isotropy of lateral resolution in coherent structured illumination microscopy," Biomed. Opt. Express 5, 1895-1912 (2014)

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