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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 7 — Jul. 1, 2014
  • pp: 2054–2065

Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes

Ann-Kathrin Kniggendorf, Merve Meinhardt-Wollweber, Xiaogang Yuan, Bernhard Roth, Astrid Seifert, Niels Fertig, and Carsten Zeilinger  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 7, pp. 2054-2065 (2014)

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The temperature-sensitive gating of human Connexin 26 (hCx26) was analyzed with confocal Raman microscopy. High-resolution Raman spectra covering the spectral range between 400 and 1500 rel. cm−1 with a spectral resolution of 1 cm−1 were fully annotated, revealing notable differences between the spectrum recorded from solubilized hCx26 in Ca2+-buffered POPC at 10°C and any other set of protein conditions (temperature, Ca2+ presence, POPC presence). Spectral components originating from specific amino acids show that the TM1/EL1 parahelix and probably the TM4 trans-membrane helix and the plug domain are involved in the gating process responsible for fully closing the hemichannel.

© 2014 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(180.5655) Microscopy : Raman microscopy

ToC Category:
Spectroscopic Diagnostics

Original Manuscript: February 28, 2014
Revised Manuscript: May 5, 2014
Manuscript Accepted: May 5, 2014
Published: June 6, 2014

Ann-Kathrin Kniggendorf, Merve Meinhardt-Wollweber, Xiaogang Yuan, Bernhard Roth, Astrid Seifert, Niels Fertig, and Carsten Zeilinger, "Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes," Biomed. Opt. Express 5, 2054-2065 (2014)

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