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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 7 — Jul. 1, 2014
  • pp: 2082–2090

Biomolecule-to-fluorescent-color encoder: modulation of fluorescence emission via DNA structural changes

Takahiro Nishimura, Yusuke Ogura, Kenji Yamada, Yuko Ohno, and Jun Tanida  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 7, pp. 2082-2090 (2014)

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A biomolecule-to-fluorescent-color (B/F) encoder for optical readout of biomolecular information is proposed. In the B/F encoder, a set of fluorescence wavelengths and their intensity levels are used for coding of a biomolecular signal. A hybridization chain reaction of hairpin DNAs labeled with fluorescent reporters was performed to generate the fluorescence color codes. The fluorescence is modulated via fluorescence resonance energy transfer, which is controlled by DNA structural changes. The results demonstrate that fluorescent color codes can be configured based on two wavelengths and five intensities using the B/F encoder, and the assigned codes can be retrieved via fluorescence measurements.

© 2014 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:
Biosensors and Molecular Diagnostics

Original Manuscript: February 14, 2014
Revised Manuscript: May 21, 2014
Manuscript Accepted: June 2, 2014
Published: June 9, 2014

Takahiro Nishimura, Yusuke Ogura, Kenji Yamada, Yuko Ohno, and Jun Tanida, "Biomolecule-to-fluorescent-color encoder: modulation of fluorescence emission via DNA structural changes," Biomed. Opt. Express 5, 2082-2090 (2014)

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