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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 7 — Jul. 1, 2014
  • pp: 2317–2332

Integral-geometry characterization of photobiomodulation effects on retinal vessel morphology

Marconi Barbosa, Riccardo Natoli, Kriztina Valter, Jan Provis, and Ted Maddess  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 7, pp. 2317-2332 (2014)

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The morphological characterization of quasi-planar structures represented by gray-scale images is challenging when object identification is sub-optimal due to registration artifacts. We propose two alternative procedures that enhances object identification in the integral-geometry morphological image analysis (MIA) framework. The first variant streamlines the framework by introducing an active contours segmentation process whose time step is recycled as a multi-scale parameter. In the second variant, we used the refined object identification produced in the first variant to perform the standard MIA with exact dilation radius as multi-scale parameter. Using this enhanced MIA we quantify the extent of vaso-obliteration in oxygen-induced retinopathic vascular growth, the preventative effect (by photobiomodulation) of exposure during tissue development to near-infrared light (NIR, 670 nm), and the lack of adverse effects due to exposure to NIR light.

© 2014 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(330.5020) Vision, color, and visual optics : Perception psychology

ToC Category:
Image Processing

Original Manuscript: April 17, 2014
Revised Manuscript: June 11, 2014
Manuscript Accepted: June 11, 2014
Published: June 18, 2014

Marconi Barbosa, Riccardo Natoli, Kriztina Valter, Jan Provis, and Ted Maddess, "Integral-geometry characterization of photobiomodulation effects on retinal vessel morphology," Biomed. Opt. Express 5, 2317-2332 (2014)

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