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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 7 — Jul. 1, 2014
  • pp: 2376–2389

Multiplexed coded illumination for Fourier Ptychography with an LED array microscope

Lei Tian, Xiao Li, Kannan Ramchandran, and Laura Waller  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 7, pp. 2376-2389 (2014)
http://dx.doi.org/10.1364/BOE.5.002376


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Abstract

Fourier Ptychography is a new computational microscopy technique that achieves gigapixel images with both wide field of view and high resolution in both phase and amplitude. The hardware setup involves a simple replacement of the microscope’s illumination unit with a programmable LED array, allowing one to flexibly pattern illumination angles without any moving parts. In previous work, a series of low-resolution images was taken by sequentially turning on each single LED in the array, and the data were then combined to recover a bandwidth much higher than the one allowed by the original imaging system. Here, we demonstrate a multiplexed illumination strategy in which multiple randomly selected LEDs are turned on for each image. Since each LED corresponds to a different area of Fourier space, the total number of images can be significantly reduced, without sacrificing image quality. We demonstrate this method experimentally in a modified commercial microscope. Compared to sequential scanning, our multiplexed strategy achieves similar results with approximately an order of magnitude reduction in both acquisition time and data capture requirements.

© 2014 Optical Society of America

OCIS Codes
(100.5070) Image processing : Phase retrieval
(170.0180) Medical optics and biotechnology : Microscopy
(170.1630) Medical optics and biotechnology : Coded aperture imaging
(110.1758) Imaging systems : Computational imaging
(110.3010) Imaging systems : Image reconstruction techniques

ToC Category:
Microscopy

History
Original Manuscript: May 9, 2014
Revised Manuscript: June 12, 2014
Manuscript Accepted: June 13, 2014
Published: June 19, 2014

Citation
Lei Tian, Xiao Li, Kannan Ramchandran, and Laura Waller, "Multiplexed coded illumination for Fourier Ptychography with an LED array microscope," Biomed. Opt. Express 5, 2376-2389 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-7-2376


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