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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 7 — Jul. 1, 2014
  • pp: 2390–2396

Multi-modal label-free imaging based on a femtosecond fiber laser

Ruxin Xie, Jue Su, Eric C. Rentchler, Ziyan Zhang, Carey K. Johnson, Honglian Shi, and Rongqing Hui  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 7, pp. 2390-2396 (2014)
http://dx.doi.org/10.1364/BOE.5.002390


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Abstract

We demonstrate multi-mode microscopy based on a single femtosecond fiber laser. Coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and photothermal images can be obtained simultaneously with this simplified setup. Distributions of lipid and hemoglobin in sliced mouse brain samples and blood cells are imaged. The dependency of signal amplitude on the pump power and pump modulation frequency is characterized, which allows to isolate the impact from different contributions.

© 2014 Optical Society of America

OCIS Codes
(140.3510) Lasers and laser optics : Lasers, fiber
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(300.6430) Spectroscopy : Spectroscopy, photothermal
(300.6450) Spectroscopy : Spectroscopy, Raman
(180.4315) Microscopy : Nonlinear microscopy
(060.5295) Fiber optics and optical communications : Photonic crystal fibers

ToC Category:
Microscopy

History
Original Manuscript: April 21, 2014
Revised Manuscript: May 19, 2014
Manuscript Accepted: May 31, 2014
Published: June 20, 2014

Citation
Ruxin Xie, Jue Su, Eric C. Rentchler, Ziyan Zhang, Carey K. Johnson, Honglian Shi, and Rongqing Hui, "Multi-modal label-free imaging based on a femtosecond fiber laser," Biomed. Opt. Express 5, 2390-2396 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-7-2390


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