OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 7 — Jul. 1, 2014
  • pp: 2405–2419

In-vivo imaging of psoriatic lesions with polarization multispectral dermoscopy and multiphoton microscopy

Dimitrios Kapsokalyvas, Riccardo Cicchi, Nicola Bruscino, Domenico Alfieri, Francesca Prignano, Daniela Massi, Torello Lotti, and Francesco S. Pavone  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 7, pp. 2405-2419 (2014)
http://dx.doi.org/10.1364/BOE.5.002405


View Full Text Article

Enhanced HTML    Acrobat PDF (6108 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Psoriasis is a skin autoimmune disease characterized by hyperkeratosis, hyperproliferation of the epidermis and dilatation of dermal papillary blood vessels. Healthy skin (5 volunteers) and psoriatic lesions (3 patients) were visualized in vivo, with high contrast and resolution, with a Polarization Multispectral Dermoscope and a Multiphoton Microscope. Psoriatic features were identified and quantified. The effective diameter of the superficial blood vessels was measured at 35.2 ± 7.2 μm and the elongated dermal papillae had an effective diameter of 64.2 ± 22.6 μm. The methodologies developed could be employed for quantitative diagnostic purposes and furthermore serve as a monitoring method of the effect of personalized treatments.

© 2014 Optical Society of America

OCIS Codes
(100.2980) Image processing : Image enhancement
(170.1870) Medical optics and biotechnology : Dermatology
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Dermatological Applications

History
Original Manuscript: February 11, 2014
Revised Manuscript: April 25, 2014
Manuscript Accepted: April 28, 2014
Published: June 24, 2014

Citation
Dimitrios Kapsokalyvas, Riccardo Cicchi, Nicola Bruscino, Domenico Alfieri, Francesca Prignano, Daniela Massi, Torello Lotti, and Francesco S. Pavone, "In-vivo imaging of psoriatic lesions with polarization multispectral dermoscopy and multiphoton microscopy," Biomed. Opt. Express 5, 2405-2419 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-7-2405


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. K. Wolff and T. B. Fitzpatrick, Fitzpatrick's Dermatology in General Medicine, 7th ed. Klaus Wolff, ed. (McGraw-Hill, 2008), pp. 2 v. (xxxv, 2402, 2136 p.).
  2. R. G. Langley, G. G. Krueger, and C. E. Griffiths, “Psoriasis: epidemiology, clinical features, and quality of life,” Ann Rheum Dis 64 Suppl 2, ii18–23; discussion ii24–15 (2005).
  3. M. A. Lowes, A. M. Bowcock, and J. G. Krueger, “Pathogenesis and therapy of psoriasis,” Nature445(7130), 866–873 (2007). [CrossRef] [PubMed]
  4. D. Kapsokalyvas, N. Bruscino, D. Alfieri, V. de Giorgi, G. Cannarozzo, R. Cicchi, D. Massi, N. Pimpinelli, and F. S. Pavone, “Spectral morphological analysis of skin lesions with a polarization multispectral dermoscope,” Opt. Express21(4), 4826–4840 (2013). [CrossRef] [PubMed]
  5. B. Masters and P. So, “Confocal microscopy and multi-photon excitation microscopy of human skin in vivo,” Opt. Express8(1), 2–10 (2001). [CrossRef] [PubMed]
  6. H. G. Breunig, H. Studier, and K. König, “Multiphoton excitation characteristics of cellular fluorophores of human skin in vivo,” Opt. Express18(8), 7857–7871 (2010). [CrossRef] [PubMed]
  7. J. A. Palero, H. S. de Bruijn, A. van der Ploeg van den Heuvel, H. J. Sterenborg, and H. C. Gerritsen, “Spectrally resolved multiphoton imaging of in vivo and excised mouse skin tissues,” Biophys. J.93(3), 992–1007 (2007). [CrossRef] [PubMed]
  8. R. Patalay, C. Talbot, Y. Alexandrov, I. Munro, M. A. Neil, K. König, P. M. French, A. Chu, G. W. Stamp, and C. Dunsby, “Quantification of cellular autofluorescence of human skin using multiphoton tomography and fluorescence lifetime imaging in two spectral detection channels,” Biomed. Opt. Express2(12), 3295–3308 (2011). [CrossRef] [PubMed]
  9. K. König, “Clinical multiphoton tomography,” J. Biophotonics1(1), 13–23 (2008). [CrossRef] [PubMed]
  10. E. Dimitrow, I. Riemann, A. Ehlers, M. J. Koehler, J. Norgauer, P. Elsner, K. König, and M. Kaatz, “Spectral fluorescence lifetime detection and selective melanin imaging by multiphoton laser tomography for melanoma diagnosis,” Exp. Dermatol.18(6), 509–515 (2009). [CrossRef] [PubMed]
  11. R. Cicchi, D. Kapsokalyvas, M. Troiano, P. Campolmi, C. Morini, D. Massi, G. Cannarozzo, T. Lotti, and F. S. Pavone, “In vivo non-invasive monitoring of collagen remodelling by two-photon microscopy after micro-ablative fractional laser resurfacing,” J. Biophotonics (2013) DOI . [CrossRef]
  12. R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010). [CrossRef] [PubMed]
  13. M. J. Koehler, A. Preller, P. Elsner, K. König, U. C. Hipler, and M. Kaatz, “Non-invasive evaluation of dermal elastosis by in vivo multiphoton tomography with autofluorescence lifetime measurements,” Exp. Dermatol.21(1), 48–51 (2012). [CrossRef] [PubMed]
  14. R. Cicchi, S. Sestini, V. De Giorgi, D. Massi, T. Lotti, and F. S. Pavone, “Nonlinear laser imaging of skin lesions,” J. Biophotonics1(1), 62–73 (2008). [CrossRef] [PubMed]
  15. R. R. Anderson, “Polarized light examination and photography of the skin,” Arch. Dermatol.127(7), 1000–1005 (1991). [CrossRef] [PubMed]
  16. S. G. Demos and R. R. Alfano, “Optical polarization imaging,” Appl. Opt.36(1), 150–155 (1997). [CrossRef] [PubMed]
  17. J. Malvehy, Handbook of Dermoscopy (Taylor & Francis, 2006), p. 95
  18. S. L. Jacques, J. C. Ramella-Roman, and K. Lee, “Imaging skin pathology with polarized light,” J. Biomed. Opt.7(3), 329–340 (2002). [CrossRef] [PubMed]
  19. Y. K. Başaran, M. S. Gürel, A. T. Erdemir, E. Turan, N. Yurt, and I. S. Bağci, “Evaluation of the response to treatment of psoriasis vulgaris with reflectance confocal microscopy,” Skin Res. Technol. (2014), doi:. [CrossRef] [PubMed]
  20. E. A. Wolberink, P. E. van Erp, R. T. de Boer-van Huizen, P. C. van de Kerkhof, and M. J. Gerritsen, “Reflectance confocal microscopy: an effective tool for monitoring ultraviolet B phototherapy in psoriasis,” Br. J. Dermatol.167(2), 396–403 (2012). [CrossRef] [PubMed]
  21. B. R. Masters, P. T. So, and E. Gratton, “Multiphoton excitation microscopy of in vivo human skin. Functional and morphological optical biopsy based on three-dimensional imaging, lifetime measurements and fluorescence spectroscopy,” Ann. N. Y. Acad. Sci.838(1 ADVANCES IN O), 58–67 (1998). [CrossRef] [PubMed]
  22. L. H. Laiho, S. Pelet, T. M. Hancewicz, P. D. Kaplan, and P. T. So, “Two-photon 3-D mapping of ex vivo human skin endogenous fluorescence species based on fluorescence emission spectra,” J. Biomed. Opt.10(2), 024016 (2005). [CrossRef] [PubMed]
  23. B. Schmid, J. Schindelin, A. Cardona, M. Longair, and M. Heisenberg, “A high-level 3D visualization API for Java and ImageJ,” BMC Bioinformatics11(1), 274 (2010). [CrossRef] [PubMed]
  24. S. Hern, A. W. Stanton, R. H. Mellor, C. C. Harland, J. R. Levick, and P. S. Mortimer, “In vivo quantification of the structural abnormalities in psoriatic microvessels before and after pulsed dye laser treatment,” Br. J. Dermatol.152(3), 505–511 (2005). [CrossRef] [PubMed]
  25. J. Hegyi, V. Hegyi, G. Messer, P. Arenberger, T. Ruzicka, and C. Berking, “Confocal laser-scanning capillaroscopy: a novel approach to the analysis of skin capillaries in vivo,” Skin Res. Technol.15(4), 476–481 (2009). [CrossRef] [PubMed]
  26. S. González, M. Rajadhyaksha, G. Rubinstein, and R. R. Anderson, “Characterization of psoriasis in vivo by reflectance confocal microscopy,” J. Med.30(5-6), 337–356 (1999). [PubMed]
  27. M. Ardigo, C. Cota, E. Berardesca, and S. González, “Concordance between in vivo reflectance confocal microscopy and histology in the evaluation of plaque psoriasis,” J. Eur. Acad. Dermatol. Venereol.23(6), 660–667 (2009). [CrossRef] [PubMed]
  28. E. A. Wolberink, P. E. van Erp, M. M. Teussink, P. C. van de Kerkhof, and M. J. Gerritsen, “Cellular features of psoriatic skin: imaging and quantification using in vivo reflectance confocal microscopy,” Cytometry B Clin. Cytom.80(3), 141–149 (2011). [CrossRef] [PubMed]
  29. R. Archid, A. Patzelt, B. Lange-Asschenfeldt, S. S. Ahmad, M. Ulrich, E. Stockfleth, S. Philipp, W. Sterry, and J. Lademann, “Confocal laser-scanning microscopy of capillaries in normal and psoriatic skin,” J. Biomed. Opt.17(10), 101511 (2012). [CrossRef] [PubMed]
  30. K. König, H. G. Breunig, R. Bückle, M. Kellner-Höfer, M. Weinigel, E. Büttner, W. Sterry, and J. Lademann, “Optical skin biopsies by clinical CARS and multiphoton fluorescence/SHG tomography,” Laser Phys. Lett.8(6), 465–468 (2011). [CrossRef]
  31. H. G. Breunig, R. Bückle, M. Kellner-Höfer, M. Weinigel, J. Lademann, W. Sterry, and K. König, “Combined in vivo multiphoton and CARS imaging of healthy and disease-affected human skin,” Microsc. Res. Tech.75(4), 492–498 (2012). [CrossRef] [PubMed]
  32. E. Ayroldi, A. Bastianelli, L. Cannarile, M. G. Petrillo, D. V. Delfino, and A. Fierabracci, “A pathogenetic approach to autoimmune skin disease therapy: psoriasis and biological drugs, unresolved issues, and future directions,” Curr. Pharm. Des.17(29), 3176–3190 (2011). [CrossRef] [PubMed]
  33. E. D. O. Roberson and A. M. Bowcock, “Psoriasis genetics: breaking the barrier,” Trends Genet.26(9), 415–423 (2010). [CrossRef] [PubMed]
  34. W. Weger, “Current status and new developments in the treatment of psoriasis and psoriatic arthritis with biological agents,” Br. J. Pharmacol.160(4), 810–820 (2010). [CrossRef] [PubMed]
  35. P. I. Spuls, L. L. A. Lecluse, M. L. N. F. Poulsen, J. D. Bos, R. S. Stern, and T. Nijsten, “How Good Are Clinical Severity and Outcome Measures for Psoriasis?: Quantitative Evaluation in a Systematic Review,” J. Invest. Dermatol.130(4), 933–943 (2010). [CrossRef] [PubMed]
  36. M. Suárez-Fariñas, K. R. Shah, A. S. Haider, J. G. Krueger, and M. A. Lowes, “Personalized medicine in psoriasis: developing a genomic classifier to predict histological response to Alefacept,” BMC Dermatol.10, 1 (2010). [PubMed]
  37. R. T. Woolf and C. H. Smith, “How genetic variation affects patient response and outcome to therapy for psoriasis,” Expert Rev. Clin. Immunol.6(6), 957–966 (2010). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4 Fig. 5
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited