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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 7 — Jul. 1, 2014
  • pp: 2420–2434

An effective nanosensor for organic molecules based on water-soluble mercaptopropionic acid-capped CdTe nanocrystals with potential application in high-throughput screening and high-resolution optical microscopy

Pick-Chung Lau, Robert A. Norwood, Masud Mansuripur, and Nasser Peyghambarian  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 7, pp. 2420-2434 (2014)
http://dx.doi.org/10.1364/BOE.5.002420


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Abstract

Specially-treated glass substrates coated with a thin film of water soluble mercaptopropionic acid (MPA) capped CdTe nanocrystals (NCs) were prepared and found to undergo photoluminescence changes by as much as 40% when micro-droplets of organic molecules were placed in the nanometer-range proximity of the NCs. This imaging technique involving close proximity between a nano-crystal and an organic molecule is found to provide a 2 × –3 × enhanced contrast ratio over the conventional method of fluorescence imaging. Photoluminescence of NCs is recoverable upon removal of the organic molecules, therefore validating these NCs as potential all-optical organic molecular nanosensors. Upon optimization and with proper instrumentation, these nano-crystals could eventually serve as point-detectors for purposes of super-resolution optical microscopy. No solvents are required for the proposed sensing mechanism since all solutions were dried under argon flow. Fluorophores and fluorescent proteins were investigated, including fluorescein, Rhodamine 6G, and green fluorescent protein (GFP). Furthermore, NC photoluminescence changes were systematically quantified as a function of the solution pH and of the organic molecule concentration. Long duration (> 40 minutes) continuous excitation studies were conducted in order to evaluate the reliability of the proposed sensing scheme.

© 2014 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(160.4236) Materials : Nanomaterials

ToC Category:
Nanotechnology and Plasmonics

History
Original Manuscript: April 11, 2014
Revised Manuscript: June 14, 2014
Manuscript Accepted: June 14, 2014
Published: June 24, 2014

Citation
Pick-Chung Lau, Robert A. Norwood, Masud Mansuripur, and Nasser Peyghambarian, "An effective nanosensor for organic molecules based on water-soluble mercaptopropionic acid-capped CdTe nanocrystals with potential application in high-throughput screening and high-resolution optical microscopy," Biomed. Opt. Express 5, 2420-2434 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-7-2420


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