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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 7 — Jul. 1, 2014
  • pp: 2420–2434

An effective nanosensor for organic molecules based on water-soluble mercaptopropionic acid-capped CdTe nanocrystals with potential application in high-throughput screening and high-resolution optical microscopy

Pick-Chung Lau, Robert A. Norwood, Masud Mansuripur, and Nasser Peyghambarian  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 7, pp. 2420-2434 (2014)

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Specially-treated glass substrates coated with a thin film of water soluble mercaptopropionic acid (MPA) capped CdTe nanocrystals (NCs) were prepared and found to undergo photoluminescence changes by as much as 40% when micro-droplets of organic molecules were placed in the nanometer-range proximity of the NCs. This imaging technique involving close proximity between a nano-crystal and an organic molecule is found to provide a 2 × –3 × enhanced contrast ratio over the conventional method of fluorescence imaging. Photoluminescence of NCs is recoverable upon removal of the organic molecules, therefore validating these NCs as potential all-optical organic molecular nanosensors. Upon optimization and with proper instrumentation, these nano-crystals could eventually serve as point-detectors for purposes of super-resolution optical microscopy. No solvents are required for the proposed sensing mechanism since all solutions were dried under argon flow. Fluorophores and fluorescent proteins were investigated, including fluorescein, Rhodamine 6G, and green fluorescent protein (GFP). Furthermore, NC photoluminescence changes were systematically quantified as a function of the solution pH and of the organic molecule concentration. Long duration (> 40 minutes) continuous excitation studies were conducted in order to evaluate the reliability of the proposed sensing scheme.

© 2014 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(160.4236) Materials : Nanomaterials

ToC Category:
Nanotechnology and Plasmonics

Original Manuscript: April 11, 2014
Revised Manuscript: June 14, 2014
Manuscript Accepted: June 14, 2014
Published: June 24, 2014

Pick-Chung Lau, Robert A. Norwood, Masud Mansuripur, and Nasser Peyghambarian, "An effective nanosensor for organic molecules based on water-soluble mercaptopropionic acid-capped CdTe nanocrystals with potential application in high-throughput screening and high-resolution optical microscopy," Biomed. Opt. Express 5, 2420-2434 (2014)

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  1. R. Elghanian, J. J. Storhoff, R. C. Mucic, R. L. Letsinger, and C. A. Mirkin, “Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles,” Science277(5329), 1078–1081 (1997). [CrossRef] [PubMed]
  2. A. R. Clapp, I. L. Medintz, J. M. Mauro, B. R. Fisher, M. G. Bawendi, and H. Mattoussi, “Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors,” J. Am. Chem. Soc.126(1), 301–310 (2004). [CrossRef] [PubMed]
  3. Y. Nagasaki, T. Ishii, Y. Sunaga, Y. Watanabe, H. Otsuka, and K. Kataoka, “Novel molecular recognition via fluorescent resonance energy transfer using a biotin-PEG/polyamine stabilized CdS quantum dot,” Langmuir20(15), 6396–6400 (2004). [CrossRef] [PubMed]
  4. K. Aslan, J. R. Lakowicz, and C. D. Geddes, “Nanogold plasmon resonance-based glucose sensing. 2. Wavelength-ratiometric resonance light scattering,” Anal. Chem.77(7), 2007–2014 (2005). [CrossRef] [PubMed]
  5. K. Aslan, C. C. Luhrs, and V. H. Pérez-Luna, “Controlled and Reversible Aggregation of Biotinylated Gold Nanoparticles with Streptavidin,” J. Phys. Chem. B108(40), 15631–15639 (2004). [CrossRef]
  6. A. J. Haes and R. P. Van Duyne, “A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles,” J. Am. Chem. Soc.124(35), 10596–10604 (2002). [CrossRef] [PubMed]
  7. D. Roll, J. Malicka, I. Gryczynski, Z. Gryczynski, and J. R. Lakowicz, “Metallic colloid wavelength-ratiometric scattering sensors,” Anal. Chem.75(14), 3440–3445 (2003). [CrossRef] [PubMed]
  8. N. Guillot and M. L. de la Chapelle, “Lithographied nanostructures as nanosensors,” J. Nanophotonics6(1), 064506 (2012). [CrossRef]
  9. W. C. Chan and S. Nie, “Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection,” Science281(5385), 2016–2018 (1998). [CrossRef] [PubMed]
  10. J. Wang, “Biomolecule-functionalized nanowires: from nanosensors to nanocarriers,” ChemPhysChem10(11), 1748–1755 (2009). [CrossRef] [PubMed]
  11. N. I. Chalmers, R. J. Palmer, L. Du-Thumm, R. Sullivan, W. Shi, and P. E. Kolenbrander, “Use of quantum dot luminescent probes to achieve single-cell resolution of human oral bacteria in biofilms,” Appl. Environ. Microbiol.73(2), 630–636 (2007). [CrossRef] [PubMed]
  12. L. Zou, Z. Gu, N. Zhang, Y. Zhang, Z. Fang, W. Zhu, and X. Zhong, “Ultrafast synthesis of highly luminescent green- to near infrared-emitting CdTe nanocrystals in aqueous phase,” J. Mater. Chem.18(24), 2807 (2008). [CrossRef]
  13. H. C. Ko, C. T. Yuan, S. H. Lin, and J. Tang, “Blinking suppression of single quantum dots in agarose gel,” Appl. Phys. Lett.96(1), 012104 (2010). [CrossRef]
  14. W. W. Yu, L. Qu, W. Guo, and X. Peng, “Experimental Determination of the Extinction Coefficient of CdTe, CdSe, and CdS Nanocrystals,” Chem. Mater.15(14), 2854–2860 (2003). [CrossRef]
  15. J. M. Dixon, M. Taniguchi, and J. S. Lindsey, “PhotochemCAD 2: a refined program with accompanying spectral databases for photochemical calculations,” Photochem. Photobiol.81(1), 212–213 (2005). [CrossRef] [PubMed]
  16. H. Du, R.-C. A. Fuh, J. Li, L. A. Corkan, and J. S. Lindsey, “PhotochemCAD: A Computer-Aided Design and Research Tool in Photochemistry,” Photochem. Photobiol.68(2), 141–142 (1998).
  17. E. Oh, M.-Y. Hong, D. Lee, S.-H. Nam, H. C. Yoon, and H.-S. Kim, “Inhibition assay of biomolecules based on fluorescence resonance energy transfer (FRET) between quantum dots and gold nanoparticles,” J. Am. Chem. Soc.127(10), 3270–3271 (2005). [CrossRef] [PubMed]
  18. R. Sjöback, J. Nygren, and M. Kubista, “Absorption and fluorescence properties of fluorescein,” Spectrochim. Acta, Part A51(6), L7–L21 (1995). [CrossRef]
  19. F. Koberling, A. Mews, and T. Basché, “Oxygen-Induced Blinking of Single CdSe Nanocrystals,” Adv. Mater.13(9), 672–676 (2001). [CrossRef]
  20. P. C. Lau, R. A. Norwood, M. Mansuripur, and N. Peyghambarian, “An effective and simple oxygen nanosensor made from MPA-capped water soluble CdTe nanocrystals,” Nanotechnology24(1), 015501 (2013). [CrossRef] [PubMed]
  21. W. G. J. H. M. van Sark, P. L. T. M. Frederix, D. J. Van den Heuvel, H. C. Gerritsen, A. A. Bol, J. N. J. van Lingen, C. de Mello Donegá, and A. Meijerink, “Photooxidation and Photobleaching of Single CdSe/ZnS Quantum Dots Probed by Room-Temperature Time-Resolved Spectroscopy,” J. Phys. Chem. B105(35), 8281–8284 (2001). [CrossRef]
  22. H. Morise, O. Shimomura, F. H. Johnson, and J. Winant, “Intermolecular energy transfer in the bioluminescent system of Aequorea,” Biochemistry13(12), 2656–2662 (1974). [CrossRef] [PubMed]

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