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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 8 — Aug. 1, 2014
  • pp: 2662–2678

In vivo mouse fluorescence imaging for folate-targeted delivery and release kinetics

Esther H. R. Tsai, Brian Z. Bentz, Venkatesh Chelvam, Vaibhav Gaind, Kevin J. Webb, and Philip S. Low  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 8, pp. 2662-2678 (2014)

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Many cancer cells over-express folate receptors, and this provides an opportunity for both folate-targeted fluorescence imaging and the development of targeted anti-cancer drugs. We present an optical imaging modality that allows for the monitoring and evaluation of drug delivery and release through disulfide bond reduction inside a tumor in vivo for the first time. A near-infrared folate-targeting fluorophore pair was synthesized and used to image a xenograft tumor grown from KB cells in a live mouse. The in vivo results are shown to be in agreement with previous in vitro studies, confirming the validity and feasibility of our method as an effective tool for preclinical studies in drug development.

© 2014 Optical Society of America

OCIS Codes
(100.3190) Image processing : Inverse problems
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Small Animal Imaging and Veterinary Studies

Esther H. R. Tsai, Brian Z. Bentz, Venkatesh Chelvam, Vaibhav Gaind, Kevin J. Webb, and Philip S. Low, "In vivo mouse fluorescence imaging for folate-targeted delivery and release kinetics," Biomed. Opt. Express 5, 2662-2678 (2014)

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