OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 8 — Aug. 1, 2014
  • pp: 2679–2685

In vivo quantitative photoacoustic microscopy of gold nanostar kinetics in mouse organs

Weitao Li, Xiaolian Sun, Yu Wang, Gang Niu, Xiaoyuan Chen, Zhiyu Qian, and Liming Nie  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 8, pp. 2679-2685 (2014)

View Full Text Article

Enhanced HTML    Acrobat PDF (2474 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We developed a high-resolution photoacoustic microscopy (PAM) system with a near-infrared (NIR) laser to noninvasively monitor the distribution of gold nanostar (GNS) in blood vessels, liver and spleen in mice. Photoacoustic images of organs at deep depths were continuously acquired in vivo every 30 minutes after a single dose of GNS by tail vein injection. The experimental results showed that GNS accumulated significantly in both liver and spleen from blood circulation after administration, which was qualitatively validated by fluorescence imaging. Our studies demonstrate that PAM might be potentially used for noninvasive tracing the kinetics of exogenous nanoparticles in biological system.

© 2014 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(110.5125) Imaging systems : Photoacoustics

ToC Category:
Photoacoustic Imaging and Spectroscopy

Original Manuscript: May 7, 2014
Revised Manuscript: June 3, 2014
Manuscript Accepted: July 11, 2014
Published: July 17, 2014

Weitao Li, Xiaolian Sun, Yu Wang, Gang Niu, Xiaoyuan Chen, Zhiyu Qian, and Liming Nie, "In vivo quantitative photoacoustic microscopy of gold nanostar kinetics in mouse organs," Biomed. Opt. Express 5, 2679-2685 (2014)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. P. C. Li, C. W. Wei, C.-K. Liao, C.-D. Chen, K.-C. Pao, C.-R. Wang, Y.-N. Wu, and D.-B. Shieh, “Photoacoustic imaging of multiple targets using gold nanorods,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control54(8), 1642–1647 (2007). [CrossRef] [PubMed]
  2. C. Kim, C. Favazza, and L. V. Wang, “In vivo photoacoustic tomography of chemicals: high-resolution functional and molecular optical imaging at new depths,” Chem. Rev.110(5), 2756–2782 (2010). [CrossRef] [PubMed]
  3. S. K. Balasubramanian, J. Jittiwat, J. Manikandan, C.-N. Ong, L. E. Yu, and W. Y. Ong, “Biodistribution of gold nanoparticles and gene expression changes in the liver and spleen after intravenous administration in rats,” Biomaterials31(8), 2034–2042 (2010). [CrossRef] [PubMed]
  4. H. Chen, A. Dorrigan, S. Saad, D. J. Hare, M. B. Cortie, and S. M. Valenzuela, “In vivo study of spherical gold nanoparticles: inflammatory effects and distribution in mice,” PLoS ONE8(2), e58208 (2013). [CrossRef] [PubMed]
  5. C. Pérez-Campaña, V. Gómez-Vallejo, M. Puigivila, A. Martín, T. Calvo-Fernández, S. E. Moya, R. F. Ziolo, T. Reese, and J. Llop, “Biodistribution of different sized nanoparticles assessed by positron emission tomography: a general strategy for direct activation of metal oxide particles,” ACS Nano7(4), 3498–3505 (2013). [CrossRef] [PubMed]
  6. C. H. Lee, S. H. Cheng, Y. J. Wang, Y. C. Chen, N. T. Chen, J. Souris, C. T. Chen, C. Y. Mou, C. S. Yang, and L. W. Lo, “Near‐Infrared Mesoporous Silica Nanoparticles for Optical Imaging: Characterization and In Vivo Biodistribution,” Adv. Funct. Mater.19(2), 215–222 (2009). [CrossRef]
  7. S. Ye, R. Yang, J. Xiong, K. K. Shung, Q. Zhou, C. Li, and Q. Ren, “Label-free imaging of zebrafish larvae in vivo by photoacoustic microscopy,” Biomed. Opt. Express3(2), 360–365 (2012). [CrossRef] [PubMed]
  8. S. Hu, K. Maslov, and L. V. Wang, “Noninvasive label-free imaging of microhemodynamics by optical-resolution photoacoustic microscopy,” Opt. Express17(9), 7688–7693 (2009). [CrossRef] [PubMed]
  9. L. Xi, C. Duan, H. Xie, and H. Jiang, “Miniature probe combining optical-resolution photoacoustic microscopy and optical coherence tomography for in vivo microcirculation study,” Appl. Opt.52(9), 1928–1931 (2013). [CrossRef] [PubMed]
  10. L. Nie, X. Cai, K. Maslov, A. Garcia-Uribe, M. A. Anastasio, and L. V. Wang, “Photoacoustic tomography through a whole adult human skull with a photon recycler,” J. Biomed. Opt.17(11), 110506 (2012). [CrossRef] [PubMed]
  11. L. Nie, Z. Guo, and L. V. Wang, “Photoacoustic tomography of monkey brain using virtual point ultrasonic transducers,” J. Biomed. Opt.16(7), 076005 (2011). [CrossRef] [PubMed]
  12. K. H. Song and L. V. Wang, “Noninvasive photoacoustic imaging of the thoracic cavity and the kidney in small and large animals,” Med. Phys.35(10), 4524–4529 (2008). [CrossRef] [PubMed]
  13. L. Nie, M. Chen, X. Sun, P. Rong, N. Zheng, and X. Chen, “Palladium nanosheets as highly stable and effective contrast agents for in vivo photoacoustic molecular imaging,” Nanoscale6(3), 1271–1276 (2014). [CrossRef] [PubMed]
  14. L. Nie, S. Wang, X. Wang, P. Rong, G. Niu, P. Huang, G. Lu, and X. Chen, “Plasmonic nanostars: in vivo volumetric photoacoustic molecular angiography and therapeutic monitoring with targeted plasmonic nanostars,” Small10(8), 1585–1593 (2014).
  15. K. H. Song and L. V. Wang, “Deep reflection-mode photoacoustic imaging of biological tissue,” J. Biomed. Opt.12(6), 060503 (2007). [CrossRef] [PubMed]
  16. J. Yao and L. V. Wang, “Photoacoustic Microscopy,” Laser Photon Rev7(5), 758–778 (2013). [CrossRef] [PubMed]
  17. H. Yuan, C. G. Khoury, H. Hwang, C. M. Wilson, G. A. Grant, and T. Vo-Dinh, “Gold nanostars: surfactant-free synthesis, 3D modelling, and two-photon photoluminescence imaging,” Nanotechnology23(7), 075102 (2012). [CrossRef] [PubMed]
  18. S. Wang, P. Huang, L. Nie, R. Xing, D. Liu, Z. Wang, J. Lin, S. Chen, G. Niu, G. Lu, and X. Chen, “Single Continuous Wave Laser Induced Photodynamic/Plasmonic Photothermal Therapy Using Photosensitizer-Functionalized Gold Nanostars,” Adv. Mater.25(22), 3055–3061 (2013). [CrossRef] [PubMed]
  19. J. Laufer, D. Delpy, C. Elwell, and P. Beard, “Quantitative spatially resolved measurement of tissue chromophore concentrations using photoacoustic spectroscopy: application to the measurement of blood oxygenation and haemoglobin concentration,” Phys. Med. Biol.52(1), 141–168 (2007). [CrossRef] [PubMed]
  20. E. P. Visser, J. A. Disselhorst, M. Brom, P. Laverman, M. Gotthardt, W. J. G. Oyen, and O. C. Boerman, “Spatial resolution and sensitivity of the Inveon small-animal PET scanner,” J. Nucl. Med.50(1), 139–147 (2008). [CrossRef] [PubMed]
  21. A. Chatziioannou, Y. C. Tai, N. Doshi, and S. R. Cherry, “Detector development for microPET II: a 1 μl resolution PET scanner for small animal imaging,” Phys. Med. Biol.46(11), 2899–2910 (2001). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited