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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 8 — Aug. 1, 2014
  • pp: 2769–2784

Speckle contrast optical spectroscopy, a non-invasive, diffuse optical method for measuring microvascular blood flow in tissue

Claudia P. Valdes, Hari M. Varma, Anna K. Kristoffersen, Tanja Dragojevic, Joseph P. Culver, and Turgut Durduran  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 8, pp. 2769-2784 (2014)

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We introduce a new, non-invasive, diffuse optical technique, speckle contrast optical spectroscopy (SCOS), for probing deep tissue blood flow using the statistical properties of laser speckle contrast and the photon diffusion model for a point source. The feasibility of the method is tested using liquid phantoms which demonstrate that SCOS is capable of measuring the dynamic properties of turbid media non-invasively. We further present an in vivo measurement in a human forearm muscle using SCOS in two modalities: one with the dependence of the speckle contrast on the source-detector separation and another on the exposure time. In doing so, we also introduce crucial corrections to the speckle contrast that account for the variance of the shot and sensor dark noises.

© 2014 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.6480) Medical optics and biotechnology : Spectroscopy, speckle
(170.7050) Medical optics and biotechnology : Turbid media

ToC Category:
Speckle Imaging and Diagnostics

Original Manuscript: June 9, 2014
Revised Manuscript: July 8, 2014
Manuscript Accepted: July 10, 2014
Published: July 23, 2014

Claudia P. Valdes, Hari M. Varma, Anna K. Kristoffersen, Tanja Dragojevic, Joseph P. Culver, and Turgut Durduran, "Speckle contrast optical spectroscopy, a non-invasive, diffuse optical method for measuring microvascular blood flow in tissue," Biomed. Opt. Express 5, 2769-2784 (2014)

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