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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 8 — Aug. 1, 2014
  • pp: 2843–2855

Histology validation of mapping depth-resolved cardiac fiber orientation in fresh mouse heart using optical polarization tractography

Y. Wang, K. Zhang, N. B. Wasala, X. Yao, D. Duan, and G. Yao  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 8, pp. 2843-2855 (2014)

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Myofiber organization in cardiac muscle plays an important role in achieving normal mechanical and electrical heart functions. An imaging tool that can reveal microstructural details of myofiber organization is valuable for both basic research and clinical applications. A high-resolution optical polarization tractography (OPT) was recently developed based on Jones matrix optical coherence tomography (JMOCT). In this study, we validated the accuracy of using OPT for measuring depth-resolved fiber orientation in fresh heart samples by comparing directly with histology images. Systematic image processing algorithms were developed to register OPT with histology images. The pixel-wise differences between the two tractographic results were analyzed in details. The results indicate that OPT can accurately image depth-resolved fiber orientation in fresh heart tissues and reveal microstructural details at the histological level.

© 2014 Optical Society of America

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(230.5440) Optical devices : Polarization-selective devices
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Optical Coherence Tomography

Original Manuscript: June 9, 2014
Revised Manuscript: July 24, 2014
Manuscript Accepted: July 24, 2014
Published: July 29, 2014

Y. Wang, K. Zhang, N. B. Wasala, X. Yao, D. Duan, and G. Yao, "Histology validation of mapping depth-resolved cardiac fiber orientation in fresh mouse heart using optical polarization tractography," Biomed. Opt. Express 5, 2843-2855 (2014)

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