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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 9 — Sep. 1, 2014
  • pp: 2883–2895

Comprehensive spectral endoscopy of topically applied SERS nanoparticles in the rat esophagus

Yu W. Wang, Altaz Khan, Steven Y. Leigh, Danni Wang, Ye Chen, Daphne Meza, and Jonathan T.C. Liu  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 9, pp. 2883-2895 (2014)

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The early detection and biological investigation of esophageal cancer would benefit from the development of advanced imaging techniques to screen for the molecular changes that precede and accompany the onset of cancer. Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) have the potential to improve cancer detection and the investigation of cancer progression through the sensitive and multiplexed phenotyping of cell-surface biomarkers. Here, a miniature endoscope featuring rotational scanning and axial pull back has been developed for 2D spectral imaging of SERS NPs topically applied on the lumenal surface of the rat esophagus. Raman signals from low-pM concentrations of SERS NP mixtures are demultiplexed in real time to accurately calculate the concentration and ratio of the NPs. Ex vivo and in vivo experiments demonstrate the feasibility of topical application and imaging of multiplexed SERS NPs along the entire length of the rat esophagus.

© 2014 Optical Society of America

OCIS Codes
(110.2350) Imaging systems : Fiber optics imaging
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2680) Medical optics and biotechnology : Gastrointestinal
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(160.4236) Materials : Nanomaterials

ToC Category:
Endoscopes, Catheters and Micro-Optics

Original Manuscript: March 25, 2014
Revised Manuscript: June 11, 2014
Manuscript Accepted: July 24, 2014
Published: August 1, 2014

Yu W. Wang, Altaz Khan, Steven Y. Leigh, Danni Wang, Ye Chen, Daphne Meza, and Jonathan T.C. Liu, "Comprehensive spectral endoscopy of topically applied SERS nanoparticles in the rat esophagus," Biomed. Opt. Express 5, 2883-2895 (2014)

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