Abstract

In July 2001 we presented a new concept of integrating a micropipette within a closed microfluidic system equipped with optical tweezers and a UV-Vis spectrometer [1

A. Alrifaiy and K. Ramser, “How to integrate a micropipette into a closed microfluidic system: absorption spectra of an optically trapped erythrocyte,” Biomed. Opt. Express 2(8), 2299–2306 (2011). [CrossRef] [PubMed]

]. In Fig. 1 of that paper a schematic of the setup was illustrated. The mirrors in the figure were wrongly oriented and hence depicted as semitransparent or transparent glasses. In Fig. 1 , below, a new schematic with correctly oriented mirrors is given.

Fig. 1 Inverted microscope that incorporates the following techniques: Gastight lab-on-a-chip with an integrated micropipette coupled to a pump system, optical tweezers for 3D steering of the single cells comprising of an IR laser, a beam expander, mirrors and a dichroic mirror and an IR blocking filter to block the IR laser. UV-Vis spectrometer with an integrated optical fiber to record the oxygenation states of the RBC, CCD camera to monitor the trapping dynamics of the cells within the micro-channel system.

References

1.	A. Alrifaiy and K. Ramser, “How to integrate a micropipette into a closed microfluidic system: absorption spectra of an optically trapped erythrocyte,” Biomed. Opt. Express 2(8), 2299–2306 (2011). [CrossRef] [PubMed]

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(220.4000) Optical design and fabrication : Microstructure fabrication
(280.2490) Remote sensing and sensors : Flow diagnostics
(300.1030) Spectroscopy : Absorption
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Microfluidics

History
Original Manuscript: January 11, 2012
Manuscript Accepted: January 11, 2012
Published: January 12, 2012

Citation
Ahmed Alrifaiy and Kerstin Ramser, "The integration of a micropipette in a closed microfluidic chip with optical tweezers for investigations of single cells: erratum," Biomed. Opt. Express 3, 295-295 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-2-295

References

2011, Alrifaiy, Biomed. Opt. Express

• A. Alrifaiy and K. Ramser, “How to integrate a micropipette into a closed microfluidic system: absorption spectra of an optically trapped erythrocyte,” Biomed. Opt. Express2(8), 2299–2306 (2011). [CrossRef] [PubMed]

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Figures

Fig. 1