OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 7 — Jul. 1, 2014
  • pp: 1980–1992
« Show journal navigation

Noncontact quantitative biomechanical characterization of cardiac muscle using shear wave imaging optical coherence tomography

Shang Wang, Andrew L. Lopez, III, Yuka Morikawa, Ge Tao, Jiasong Li, Irina V. Larina, James F. Martin, and Kirill V. Larin  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 7, pp. 1980-1992 (2014)
http://dx.doi.org/10.1364/BOE.5.001980


View Full Text Article

Acrobat PDF (2116 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We report on a quantitative optical elastographic method based on shear wave imaging optical coherence tomography (SWI-OCT) for biomechanical characterization of cardiac muscle through noncontact elasticity measurement. The SWI-OCT system employs a focused air-puff device for localized loading of the cardiac muscle and utilizes phase-sensitive OCT to monitor the induced tissue deformation. Phase information from the optical interferometry is used to reconstruct 2-D depth-resolved shear wave propagation inside the muscle tissue. Cross-correlation of the displacement profiles at various spatial locations in the propagation direction is applied to measure the group velocity of the shear waves, based on which the Young’s modulus of tissue is quantified. The quantitative feature and measurement accuracy of this method is demonstrated from the experiments on tissue-mimicking phantoms with the verification using uniaxial compression test. The experiments are performed on ex vivo cardiac muscle tissue from mice with normal and genetically altered myocardium. Our results indicate this optical elastographic technique is useful as a noncontact tool to assist the cardiac muscle studies.

© 2014 Optical Society of America

1. Introduction

Heart failure after ischemic heart disease is a leading cause of death in the United States [1

1. S. L. Murphy, J. Xu, and K. D. Kochanek, “Deaths: Final Data for 2010,” Natl. Vital Stat. Rep. 61, 1–117 (2013).

]. The major limitation for the recovery from the ischemic heart disease is the poor regeneration of cardiomyocytes [2

2. M. Mercola, P. Ruiz-Lozano, and M. D. Schneider, “Cardiac muscle regeneration: lessons from development,” Genes Dev. 25(4), 299–309 (2011). [CrossRef] [PubMed]

]. A number of research groups have been working on the understanding of cardiomyosite renewal mechanisms and developing of cardiac regeneration strategies in transgenic mouse models [3

3. T. Heallen, Y. Morikawa, J. Leach, G. Tao, J. T. Willerson, R. L. Johnson, and J. F. Martin, “Hippo signaling impedes adult heart regeneration,” Development 140(23), 4683–4690 (2013). [CrossRef] [PubMed]

, 4

4. M. Xin, Y. Kim, L. B. Sutherland, M. Murakami, X. Qi, J. McAnally, E. R. Porrello, A. I. Mahmoud, W. Tan, J. M. Shelton, J. A. Richardson, H. A. Sadek, R. Bassel-Duby, and E. N. Olson, “Hippo pathway effector Yap promotes cardiac regeneration,” Proc. Natl. Acad. Sci. U.S.A. 110(34), 13839–13844 (2013). [CrossRef] [PubMed]

]. Since the heart is a dynamic organ with highly regulated function, noninvasive assessment of the localized biomechanical properties of mouse cardiac muscle is required to provide useful information regarding the potential mechanical function of the heart [5

5. H. Jawad, A. R. Lyon, S. E. Harding, N. N. Ali, and A. R. Boccaccini, “Myocardial tissue engineering,” Br. Med. Bull. 87(1), 31–47 (2008). [CrossRef] [PubMed]

, 6

6. R. M. Setser, N. G. Smedira, M. L. Lieber, E. D. Sabo, and R. D. White, “Left ventricular torsional mechanics after left ventricular reconstruction surgery for ischemic cardiomyopathy,” J. Thorac. Cardiovasc. Surg. 134(4), 888–896 (2007). [CrossRef] [PubMed]

] and thus can be an essential tool for such studies.

Atomic force microscopy (AFM) has been widely used to probe the elasticity of single cells at nano-scale spatial resolution [7

7. T. G. Kuznetsova, M. N. Starodubtseva, N. I. Yegorenkov, S. A. Chizhik, and R. I. Zhdanov, “Atomic force microscopy probing of cell elasticity,” Micron 38(8), 824–833 (2007). [CrossRef] [PubMed]

], and the quantitative elastic moduli of isolated cardiac cells are available through force spectroscopic analysis [8

8. A. B. Mathur, A. M. Collinsworth, W. M. Reichert, W. E. Kraus, and G. A. Truskey, “Endothelial, cardiac muscle and skeletal muscle exhibit different viscous and elastic properties as determined by atomic force microscopy,” J. Biomech. 34(12), 1545–1553 (2001). [CrossRef] [PubMed]

]. However, the AFM imaging protocol lacks the capability to conduct measurements in situ or in vivo. Elastographic techniques based on ultrasonic imaging and magnetic resonance imaging are also well established for in vivo detection of tissue mechanical properties [9

9. A. P. Sarvazyan, O. V. Rudenko, S. D. Swanson, J. B. Fowlkes, and S. Y. Emelianov, “Shear wave elasticity imaging: a new ultrasonic technology of medical diagnostics,” Ultrasound Med. Biol. 24(9), 1419–1435 (1998). [CrossRef] [PubMed]

, 10

10. Y. K. Mariappan, K. J. Glaser, and R. L. Ehman, “Magnetic resonance elastography: a review,” Clin. Anat. 23(5), 497–511 (2010). [CrossRef] [PubMed]

]. Based on the monitoring of shear wave propagation inside tissue, the quantification of both elasticity and viscosity can be performed with in-depth resolution. Though, the limited spatial resolution of these two imaging modalities restricts the elastographic measurement to the organ level. Thus, methods that enable biomechanical measurement at the micro scale (single or group of a few cells) with potential to perform in vivo measurements are of great importance and could lead to more thorough and direct analysis of normal, diseased, and regenerated cardiac muscle tissues and help in guiding therapies targeted into restoring cardiomyocyte activity after, e.g., myocardium infarction.

Recently, Song et al. [31

31. S. Song, Z. Huang, T. M. Nguyen, E. Y. Wong, B. Arnal, M. O’Donnell, and R. K. Wang, “Shear modulus imaging by direct visualization of propagating shear waves with phase-sensitive optical coherence tomography,” J. Biomed. Opt. 18(12), 121509 (2013). [CrossRef] [PubMed]

] and our group [37

37. S. Wang and K. V. Larin, “Shear wave imaging optical coherence tomography (SWI-OCT) for ocular tissue biomechanics,” Opt. Lett. 39(1), 41–44 (2014). [CrossRef] [PubMed]

] have developed OCE imaging method that enables depth-resolved visualization of the elastic wave at an ultra-fast frame rate. Here, we present the further development of the shear wave imaging OCT (SWI-OCT) [37

37. S. Wang and K. V. Larin, “Shear wave imaging optical coherence tomography (SWI-OCT) for ocular tissue biomechanics,” Opt. Lett. 39(1), 41–44 (2014). [CrossRef] [PubMed]

] for noncontact quantitative biomechanical characterization of mouse cardiac tissues. The group velocity of shear wave is calculated based on the cross-correlation of the tissue temporal displacement profiles at various locations in the wave propagation direction. The quantitative elasticity of sample is achieved based on the shear wave velocity through Kelvin-Voigt model. Results (Young’s modulus) from the experiments on tissue-mimicking phantoms are verified with uniaxial compression tests, which indicate the measurement accuracy of our quantitative SWI-OCT method. The feasibility of using this elastographic technique as a tool in cardiac research is demonstrated by analyzing control and mutant mouse hearts (CKO Lats1/2) with genetically altered cardiomyocytes that are expected to have lower stiffness of myocardium [3

3. T. Heallen, Y. Morikawa, J. Leach, G. Tao, J. T. Willerson, R. L. Johnson, and J. F. Martin, “Hippo signaling impedes adult heart regeneration,” Development 140(23), 4683–4690 (2013). [CrossRef] [PubMed]

]. The results suggest that this quantitative SWI-OCT method holds great potential for in situ noncontact biomechanical assessment of live heart muscle tissue during, e.g., open-chest surgery, and can greatly assist in cardiac tissue research, specifically in cardiac regeneration studies.

2. Materials and methods

2.1 Tissue-mimicking phantoms

Tissue-mimicking phantoms with known stiffness are used in this study to demonstrate the feasibility of using SWI-OCT to quantitatively measure the elasticity of soft samples. Phantoms with three different concentrations (0.50%, 0.75%, and 1.00%, weight/weight) of agar powder are made with the thickness of ~10 mm. For each concentration, three phantoms are prepared and utilized in the experiments for statistical purpose.

2.2 Ex vivo mouse cardiac muscle

To study the feasibility of using SWI-OCT for noncontact quantitative characterization of the mechanical properties of mouse cardiac muscle tissues, we performed measurements on mutant mouse hearts (CKO Lats1/2) with genetically altered cardiomyocytes, which are expected to have lower stiffness. Specifically, eight weeks to 4 months old animals of the conditional knockout line of Lats1/2 (Lats1/2F/F; Myh6-cre/Esr1*) [3

3. T. Heallen, Y. Morikawa, J. Leach, G. Tao, J. T. Willerson, R. L. Johnson, and J. F. Martin, “Hippo signaling impedes adult heart regeneration,” Development 140(23), 4683–4690 (2013). [CrossRef] [PubMed]

] are used. On days 4 and 5 before the experiments, 100 µl of 10 mg/ml tamoxifen (Sigma) in peanut oil (Sigma) is injected in each animal to activate Cre activity. Peanut oil injected animals with the same genotype are used as control. To eliminate the effect of the tissue contraction on elastographic characterization, right before the measurements, all the animals are perfused with 30 mM potassium chloride to ensure cardiac tissue relaxation. Freshly dissected pieces of the myocardium from the left ventricular wall are used for the SWI-OCT imaging. The samples were dissected as cuboids with the length of ~10 mm, the width of ~6 mm and the thickness of ~2.5 mm. The number of mice used for the analysis is three and four for the control group and the CKO Lats1/2 group, respectively. All animal manipulation procedures have been approved by the Institutional Animal Care and Use Committee of the Baylor College of Medicine.

2.3 SWI-OCT system

The SWI-OCT system combines a focused air-puff device and Fourier domain OCT [37

37. S. Wang and K. V. Larin, “Shear wave imaging optical coherence tomography (SWI-OCT) for ocular tissue biomechanics,” Opt. Lett. 39(1), 41–44 (2014). [CrossRef] [PubMed]

]. The schematic of the system is shown in Fig. 1(a)
Fig. 1 Imaging approach. (a) Schematic of SWI-OCT system setup that combines a focused air-puff device and phase-sensitive Fourier domain OCT. M-mirror; AL-achromatic lens; FC-fiber connector; FOC-fiber optics coupler; PC-polarization controller; C-collimator; SL-scan lens; TG-transmission grating; A-aperture; GM-galvanometer mirror. (b) En-face structural image from the OCT system showing the relative position and direction among the muscle fiber orientation, the air-puff port and the scanning line. The dashed lines contour the air-puff port. The SWI-OCT scanning line and the muscle fiber orientation are also called out. Scale bar corresponds to 0.5 mm.
. The home-built air-puff device provides a short-duration (Gaussian shape, 0.8 ms FWHM), low-pressure air stream on the sample surface with an excitation pressure that can be controlled and estimated based on the loading parameters [38

38. S. Wang, K. V. Larin, J. Li, S. Vantipalli, R. K. Manapuram, S. Aglyamov, S. Emelianov, and M. D. Twa, “A focused air-pulse system for optical-coherence-tomography-based measurements of tissue elasticity,” Laser Phys. Lett. 10(7), 075605 (2013). [CrossRef]

]. The phase-sensitive OCT system employs a Titanium:Sapphire laser source (Micra-5, Coherent, Inc.) with the central wavelength of ~808 nm and the bandwidth of ~110 nm. The collimated laser beam is coupled into a single-mode fiber with the setup shown in Fig. 1(a), which enables adjustable power delivered to the sample arm of the OCT system. The interference of light from the reference arm and the sample arm through a 50/50 fiber optic coupler is spectrally resolved through a high-resolution spectrometer with a CMOS camera (Basler, Inc.) running at 62.5 kHz line rate. This provides the OCT system with the temporal resolution of 16 μs for M-mode imaging. The axial resolution of the system is measured to be ~5 μm in tissue, and the FWHM of the OCT beam at focal plane is ~4 μm. The sensitivity of the system to sample displacement reaches ~11 nm (measured based on the standard deviation of the phase values over time on ex vivo cardiac muscle tissue) with the phase-resolved detection that provides sufficient SNR for the monitoring of the induced sample deformation. Also, this high sensitivity of detection makes it possible to reduce the required deformation amplitude in the sample, which helps to maintain the structural and functional properties of the sample during measurement.

2.4 Data acquisition

The SWI-OCT method enables the visualization of the elastic wave propagation at the equivalent frame rate of the A-line acquisition speed through the synchronization between the air-puff excitation and the OCT M-mode imaging [37

37. S. Wang and K. V. Larin, “Shear wave imaging optical coherence tomography (SWI-OCT) for ocular tissue biomechanics,” Opt. Lett. 39(1), 41–44 (2014). [CrossRef] [PubMed]

]. Briefly, the OCT beam controlled by a galvanometer-mirror forms a 1-D scanning line on the tissue surface covering a particular distance to monitor the elastic wave propagation. At each position of this scanning line, M-mode imaging is performed to record localized displacement profiles of the sample at all available imaging depths (up to ~0.7 mm in mouse cardiac muscle tissue). The air-puff excitation is maintained at a constant position within the scanning line and the opening of the air gate is synchronized with the start of each M-mode imaging through sharing the same triggering signal from the computer, as shown in Fig. 1(a). Thus, with respect to the air-puff loading, the detection of the sample deformation at all scanning positions is performed simultaneously, which allows depth-resolved 2-D imaging of the wave propagation at the frame rate of 62.5 kHz. Figure 1(b) shows the typical loading and imaging setup with an OCT en-face structural image of the mouse cardiac muscle from the left ventricle wall.

During experiments, all the samples are oriented similarly to have the muscle fibers aligned with the OCT beam scanning line, as shown in Fig. 1(b). The orientation of the muscle fibers is determined by visual observation under a microscope (as well as clearly seen from OCT en face images). This procedure minimizes the influence of muscle anisotropy on calculated values. The direction of the air-puff port is set perpendicular to the direction of the imaging line and the air-puff delivery angle is kept as ~20° relative to the sample surface normal for all measurements. To avoid dehydration of the tissues, at least half of the sample is submerged inside the 30 mM potassium chloride during measurements. The data acquisition with SWI-OCT for each sample takes 30 seconds. The spatial interval between adjacent scanning points is set to be ~12.5 μm.

2.5 Quantification of shear wave velocity and sample elasticity

3. Results

The shear wave velocity values are measured to be 1.01 ± 0.04 m/s, 1.89 ± 0.04 m/s, and 3.13 ± 0.09 m/s for the tissue-mimicking phantoms with the agar concentrations of 0.5%, 0.75%, and 1%, respectively. The quantification of the Young’s moduli of these samples is based on Eq. (3), and the results are presented in Fig. 3
Fig. 3 Young’s moduli quantified from SWI-OCT for the tissue-mimicking phantoms with 1%, 0.75% and 0.5% agar concentrations, compared with the results from uniaxial compression tests. Number of samples N = 3 for all measurements.
. Quantitatively, for the concentrations of 0.5%, 0.75%, and 1%, the Young’s moduli measured from SWI-OCT are 3.1 ± 0.2 kPa 10.7 ± 0.5 kPa, and 29.5 ± 1.6 kPa, respectively, which indicates the increase of the sample stiffness with the increase of the agar concentration. For the verification of these data, we also performed elasticity measurements with uniaxial compression test (using Instron Model 5943) on the tissue-mimicking phantoms with the same concentrations (number of samples N = 3). Results show the Young’s moduli of 3.8 ± 0.3 kPa, 11.0 ± 0.3 kPa, and 29.1 ± 0.4 kPa for the 0.5%, 0.75%, and 1% agar phantoms, respectively, as shown in Fig. 3. The good agreement between the SWI-OCT measurements and the uniaxial compression tests demonstrates the feasibility of using SWI-OCT for quantitative assessment of the elasticity of soft samples.

The reconstructed shear wave propagation in the typical cardiac muscle tissues from the control and the CKO Lats1/2 mice are shown in Fig. 4(a)
Fig. 4 The 2-D depth-resolved reconstruction of shear wave propagation in the cardiac muscle tissues from (a) control and (b) CKO Lats1/2 mice (Media 1). The magnitude of deformation at each spatial location is normalized to the absolute value of its minimum over time. Scale bars correspond to 0.5 mm. The red dots represent the air-puff excitation positions on both tissue samples.
and 4(b), respectively, at three representative temporal points. In both samples, the shear waves propagate from the left side to the right side, with the red dots indicating the air-puff excitation positions. The deformation magnitude at each spatial location is normalized to the absolute value of its minimum over time to provide better visualization of the shear wave propagation in the tissues. The 2-D depth-resolved images of tissue displacements are superimposed on the OCT structural images. It can be seen that over the same period of time, the shear wave propagates over a longer distance in the control sample than in the sample with genetically altered cardiomyocytes, indicating a lower shear wave velocity and thus the lower stiffness of the CKO Lats1/2 mutant cardiac tissue.

The plots of the shear wave propagation distance versus the time delay are shown in Fig. 5(a)
Fig. 5 Plots of the shear wave propagation distance versus the time delay at typical depths for the myocardium from (a) control and (b) CKO Lats1/2 mice. The magnitude of deformation at each spatial location is normalized to the absolute value of its minimum over time. The time axes indicate the shear wave propagation with the time delay forming over spatial locations.
and 5(b) for the typical cardiac muscle tissues from the control and CKO Lats1/2 mice, respectively. The dashed arrows indicate the directions of the maximum cross-correlation coefficients, showing slower shear wave propagation in the mutant muscle tissue with altered cardiomyocytes. The quantitative results are shown in Fig. 6(a)
Fig. 6 The SWI-OCT measurements reveal lower stiffness in Lats1/2 deficient cardiac tissues. (a) Shear wave velocities and (b) Young’s moduli of cardiac muscle tissues from control and CKO Lats1/2 mice. * p<0.05 from unpaired two-sample student’s t-test. Number of samples N = 3 for control and N = 4 for CKO Lats1/2 mice.
and 6(b) with the group velocities of the shear waves and the Young’s moduli of the tissues, respectively. The group velocities of the shear waves are measured to be 7.0 ± 2.1 m/s and 2.9 ± 1.3 m/s, and the Young’s moduli are quantified as 165.3 ± 89 kPa and 30.4 ± 22.6 kPa for the left ventricle cardiac muscle tissues from the control and CKO Lats1/2 mice, respectively. The unpaired two-sample student’s t-test applied on the results of Young’s moduli shows statistical significance of the difference in the elasticity of muscle tissues with normal and genetically altered cardiomyocytes. It is well known that the stiffness of the cardiac muscle tissues increases with age [42

42. A. Biernacka and N. G. Frangogiannis, “Aging and Cardiac Fibrosis,” Aging Dis. 2(2), 158–173 (2011). [PubMed]

]. The Lats1/2 deficient cardiomyocytes are thought to have more fetal-like characteristics [3

3. T. Heallen, Y. Morikawa, J. Leach, G. Tao, J. T. Willerson, R. L. Johnson, and J. F. Martin, “Hippo signaling impedes adult heart regeneration,” Development 140(23), 4683–4690 (2013). [CrossRef] [PubMed]

, 4

4. M. Xin, Y. Kim, L. B. Sutherland, M. Murakami, X. Qi, J. McAnally, E. R. Porrello, A. I. Mahmoud, W. Tan, J. M. Shelton, J. A. Richardson, H. A. Sadek, R. Bassel-Duby, and E. N. Olson, “Hippo pathway effector Yap promotes cardiac regeneration,” Proc. Natl. Acad. Sci. U.S.A. 110(34), 13839–13844 (2013). [CrossRef] [PubMed]

], suggesting lower stiffness of the hearts as compared to the control ones, which is consistent with our experimental results characterized by SWI-OCT. This suggests that the quantitative SWI-OCT method can be used to assist cardiac muscle studies with respect to the assessment of the sample mechanical properties.

The uniaxial tension test (using Instron Model 5943) was performed on the control left ventricle cardiac muscle tissues from mice of similar age. The Young’s modulus was found to be ~190 kPa, which is close to the values obtained using SWI-OCT method. Due to the inhomogeneity of the cardiac muscle tissue at the measurement scale, the applied assumption of isotropic homogeneous material for the quantitative model might introduce errors for the estimation of the true Young’s modulus. To further optimize the quantification, more sophisticated analytical model is needed describing the complexity of shear wave propagation in inhomogeneous multilayered tissues.

4. Discussions and conclusions

The described method employs the multiwave imaging principle [43

43. M. Fink and M. Tanter, “Multiwave imaging and super resolution,” Print edition 63, 28–33 (2010).

], where the imaging contrast and the imaging resolution are separately provided by two sources. Specifically, the induced shear wave inside the sample introduce the mechanical contrast that enables the measurement of the sample elasticity, and the use of OCT to monitor the shear wave propagation results in the improvement of the imaging resolution from millimeter scale (wavelength of the mechanical wave) to micron scale (coherence length of the optical wave). The wavelength of the induced shear wave could be controlled based on the loading parameters. For example, reducing the spot size of the air-puff stimulation will introduce more high frequency components to the shear wave, which will improve its capability to sense the mechanical gradient of inhomogeneous samples.

For quantitative SWI-OCT, the temporal resolution of the OCT system is an important parameter that determines the highest shear wave velocity this method can measure and also limits the transverse mapping scale for the potential heterogeneity detection. Considering a shear wave speed of 5 m/s in the sample, with the current 0.016 ms temporal resolving ability, the smallest transverse distance that allows the SWI-OCT system to monitor the wave propagation is 80 μm, which can be treated as the transverse scale for spatially mapping the elasticity of the sample. On the other hand, in terms of the measurement range, with increased transverse distance that is used for the quantification, the detectable wave velocity is proportionally increased. The implementation of a Fourier domain OCT system with 500 kHz A-line rate [44

44. L. An, P. Li, T. T. Shen, and R. Wang, “High speed spectral domain optical coherence tomography for retinal imaging at 500,000 A‑lines per second,” Biomed. Opt. Express 2(10), 2770–2783 (2011). [CrossRef] [PubMed]

] will improve the capability of this SWI-OCT method for the future heterogeneity studies of mouse cardiac muscle. The smallest wave velocity that the SWI-OCT method can cover is determinant on the recording time of each M-mode OCT imaging. Longer time of M-mode data acquisition results in extended measurable range, however, sacrificing the possibility of rapid detection. Specifically, having 0.016 ms temporal resolution, 32 ms duration of each M-mode acquisition and a typical 1mm distance, the wave velocity range that theoretically can be measured is from 31.25 mm/s to 62.5 m/s. Thus, the range of the measurable Young’s modulus is from ~3.1Pa to ~12.4 MPa.

The sensitivity of the measurement can be defined as the smallest change of the Young’s modulus that can be detected, meaning one pixel shift from the temporal scale during the velocity measurement. Based on this definition, the sensitivity of this method depends on the system temporal resolution, wave propagation distance that is used for the quantification and wave speed. For instance, with the same temporal resolution (0.016 ms) and selected distance (1 mm), the wave velocity of ~31.25 mm/s (the lowest velocity that can be measured) results in the theoretically smallest resolvable change of wave velocity as ~15.63 μm/s, and theoretically ~0.003 Pa of the change of Young’s modulus, which can be considered as the best sensitivity that can be theoretically achieved with the current system. However, practically, measurement error may occur from the estimation of the time delay due to the discrete sampling in the temporal scale, especially for the stiffer samples that have relatively higher shear wave velocity.

In SWI-OCT, the field of view of the detection is limited by the propagation distance of the shear wave. Due to the significant attenuation of the wave amplitude, in order to cover a relatively larger region, the deformation magnitude at the shear source is required to be increased, which, however, might result in unreliable measurement of the sample response using the optical phase information from low coherence interferometry [45

45. M. A. Choma, A. K. Ellerbee, C. Yang, T. L. Creazzo, and J. A. Izatt, “Spectral-domain phase microscopy,” Opt. Lett. 30(10), 1162–1164 (2005). [CrossRef] [PubMed]

]. Also, the associated higher loading pressure may alter the sample properties. Conducting multiple measurements at several locations [32

32. T.-M. Nguyen, S. Song, B. Arnal, Z. Huang, M. O’Donnell, and R. K. Wang, “Visualizing ultrasonically induced shear wave propagation using phase-sensitive optical coherence tomography for dynamic elastography,” Opt. Lett. 39(4), 838–841 (2014). [CrossRef] [PubMed]

] can be a good way to address this issue and enlarge the detection region.

During our quantification of the sample elasticity, by using Eq. (1) and (3), we also assume the negligible viscosity of the sample and do not take the dispersion of the shear wave into account for the group velocity calculation. The presented results demonstrate under such assumptions (including the ones presented in Section 2.5) the estimated Young’s moduli of soft samples using SWI-OCT agree well with the uniaxial compression measurements, which indicate the quantitative capability of SWI-OCT. Future work will be focused on the spectral analysis of the detected shear waves [46

46. S. Chen, M. W. Urban, C. Pislaru, R. Kinnick, Y. Zheng, A. Yao, and J. F. Greenleaf, “Shearwave dispersion ultrasound vibrometry (SDUV) for measuring tissue elasticity and viscosity,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control 56(1), 55–62 (2009). [CrossRef] [PubMed]

] with the quantification of phase velocity to obtain both elasticity and viscosity of the mouse cardiac muscle tissues.

Compared with AFM that has been generally used in cardiac tissue research with cellular level detection [47

47. W. Hiesinger, M. J. Brukman, R. C. McCormick, J. R. Fitzpatrick 3rd, J. R. Frederick, E. C. Yang, J. R. Muenzer, N. A. Marotta, M. F. Berry, P. Atluri, and Y. J. Woo, “Myocardial tissue elastic properties determined by atomic force microscopy after stromal cell-derived factor 1α angiogenic therapy for acute myocardial infarction in a murine model,” J. Thorac. Cardiovasc. Surg. 143(4), 962–966 (2012). [CrossRef] [PubMed]

], SWI-OCT probes the group elastic effect of the cardiomyocytes that we believe can provide new information regarding the heart biomechanics, as an important assistance for better understanding of the properties of cardiac muscle. Also, the measurement scale and the procedure of SWI-OCT enable the potential studies of myocardium elasticity under different cardiomyosite status, such as during cell contraction and with different cardiomyosite directions, which are of great importance for the mechanical tests of regenerated or engineered cardiac muscle tissues. Unlike the AFM that is difficult to be applied for in situ or in vivo detection, SWI-OCT is possible to be utilized for intraoperative measurement on live mouse, and also, the integration of this method with endoscopic techniques, such as needle OCE [15

15. K. M. Kennedy, B. F. Kennedy, R. A. McLaughlin, and D. D. Sampson, “Needle optical coherence elastography for tissue boundary detection,” Opt. Lett. 37(12), 2310–2312 (2012). [CrossRef] [PubMed]

, 48

48. R. C. Chivers, “Tissue characterization,” Ultrasound Med. Biol. 7(1), 1–20 (1981). [CrossRef] [PubMed]

], might eliminate the requirement of opening the mouse chest. Compared with the traditional ultrasound elastography or magnetic resonance elastography, SWI-OCT has superior sensitivity of detecting the tissue displacement and can be used to obtain the sample elasticity with better preservation of its structure and functions. Because OCT provides 3-D high-resolution (micro-scale) images of the tissue with its optical properties, for example, the attenuation coefficient [49

49. B. R. Klyen, L. Scolaro, T. Shavlakadze, M. D. Grounds, and D. D. Sampson, “Optical coherence tomography can assess skeletal muscle tissue from mouse models of muscular dystrophy by parametric imaging of the attenuation coefficient,” Biomed. Opt. Express 5(4), 1217–1232 (2014). [CrossRef] [PubMed]

] and the polarization property [50

50. C. Fan and G. Yao, “Imaging myocardial fiber orientation using polarization sensitive optical coherence tomography,” Biomed. Opt. Express 4(3), 460–465 (2013). [CrossRef] [PubMed]

], SWI-OCT holds the potential for multi-dimensional tissue characterization with one system.

To the best of our knowledge, this study provides the first demonstration of using quantitative OCE for noncontact biomechanical characterization of cardiac muscle and opens the door for a number of studies in the area of cardiac tissue engineering and cardiac muscle regeneration. Compared with the recently reported shear-wave-based OCE techniques which utilize piezoelectric actuator [30

30. T. M. Nguyen, S. Song, B. Arnal, E. Y. Wong, Z. Huang, R. K. Wang, and M. O’Donnell, “Shear wave pulse compression for dynamic elastography using phase-sensitive optical coherence tomography,” J. Biomed. Opt. 19(1), 016013 (2014). [CrossRef] [PubMed]

, 31

31. S. Song, Z. Huang, T. M. Nguyen, E. Y. Wong, B. Arnal, M. O’Donnell, and R. K. Wang, “Shear modulus imaging by direct visualization of propagating shear waves with phase-sensitive optical coherence tomography,” J. Biomed. Opt. 18(12), 121509 (2013). [CrossRef] [PubMed]

] and ultrasound waves [32

32. T.-M. Nguyen, S. Song, B. Arnal, Z. Huang, M. O’Donnell, and R. K. Wang, “Visualizing ultrasonically induced shear wave propagation using phase-sensitive optical coherence tomography for dynamic elastography,” Opt. Lett. 39(4), 838–841 (2014). [CrossRef] [PubMed]

, 51

51. M. Razani, T. W. H. Luk, A. Mariampillai, P. Siegler, T.-R. Kiehl, M. C. Kolios, and V. X. D. Yang, “Optical coherence tomography detection of shear wave propagation in inhomogeneous tissue equivalent phantoms and ex-vivo carotid artery samples,” Biomed. Opt. Express 5(3), 895–906 (2014). [CrossRef] [PubMed]

] for the loading of sample, SWI-OCT relies on a transient air stream to interrogate the tissue and thus requires no contact and no acoustic coupling medium for the elasticity measurement. This noncontact feature of SWI-OCT enables potentially easier application for the in situ cardiac muscle characterization with better protection of the tissue. To avoid the interference of the heartbeat motion during SWI-OCT imaging, a few potential solutions could be employed such as: (1) temporary heart stopping procedure [52

52. D. G. Melrose, B. Dreyer, H. H. Bentall, and J. B. E. Baker, “Elective Cardiac Arrest,” Lancet 266(6879), 21–23 (1955). [CrossRef] [PubMed]

], which allows the measurement performed on an arrested heart; (2) employing synchronization between the loading-measurement and the heartbeat through electrocardiogram or optical mapping; (3) using post-imaging synchronization algorithms [53

53. I. V. Larina, K. V. Larin, M. E. Dickinson, and M. Liebling, “Sequential Turning Acquisition and Reconstruction (STAR) method for four-dimensional imaging of cyclically moving structures,” Biomed. Opt. Express 3(3), 650–660 (2012). [CrossRef] [PubMed]

, 54

54. M. Liebling, A. S. Forouhar, M. Gharib, S. E. Fraser, and M. E. Dickinson, “Four-dimensional cardiac imaging in living embryos via postacquisition synchronization of nongated slice sequences,” J. Biomed. Opt. 10(5), 054001 (2005). [CrossRef] [PubMed]

], or (4) using optical pacing [55

55. M. W. Jenkins, Y. T. Wang, Y. Q. Doughman, M. Watanabe, Y. Cheng, and A. M. Rollins, “Optical pacing of the adult rabbit heart,” Biomed. Opt. Express 4(9), 1626–1635 (2013). [CrossRef] [PubMed]

]. All of these techniques can minimize the influence of the heart motion on the imaging of the shear wave propagation and improve quantification of muscle biomechanical properties.

The shear wave in cardiac muscle tissue has the frequency range of approximately 0-2.5 kHz (cut-off frequency at 20 dB drop). This results in relatively large wavelength of the shear wave (millimeter scale). Although only parts of the wavelength is presented in the field of view (shown in Fig. 4), the propagation of the wave can be clearly observed based on the time delay of the local deformation profiles and the wave group velocity can be determined through the cross-correlation approach.

Because of the air-puff stimulation, surface Rayleigh wave can also be generated. Because Rayleigh wave and shear wave have very similar wave velocities [56

56. J. Xia, R. D. Miller, and C. B. Park, “Estimation of near-surface shear-wave velocity by inversion of Rayleigh waves,” Geophysics 64(3), 691–700 (1999). [CrossRef]

], in our experiments, we have not observed distinct difference between them. Faster imaging system and larger transverse scans need to be applied in order to resolve the propagation difference between the surface Rayleigh wave and the shear wave. Our ongoing study is focused on separating these two different types of elastic waves and investigating how they can be utilized together to improve the measurement accuracy of the tissue elasticity.

In conclusion, we present a quantitative optical elastographic method based on SWI-OCT for the use of noncontact biomechanical characterization of mouse cardiac muscle tissues. The feasibility of this method in quantitatively measuring sample elasticity is verified with uniaxial compression tests on tissue-mimicking phantoms. The results from ex vivo mouse myocardium samples with normal and genetically altered cardiomyocytes demonstrate this method can be used as an essential tool in cardiac research.

Acknowledgments

This work was supported in part by the National Institute of Health (Grants R01EY022362, R01HL120140, R01HL118761 and R01DE023177), The Vivian L. Smith Foundation, and the American Heart Association (10SDG3830006 and 13POST17040027).

References and links

1.

S. L. Murphy, J. Xu, and K. D. Kochanek, “Deaths: Final Data for 2010,” Natl. Vital Stat. Rep. 61, 1–117 (2013).

2.

M. Mercola, P. Ruiz-Lozano, and M. D. Schneider, “Cardiac muscle regeneration: lessons from development,” Genes Dev. 25(4), 299–309 (2011). [CrossRef] [PubMed]

3.

T. Heallen, Y. Morikawa, J. Leach, G. Tao, J. T. Willerson, R. L. Johnson, and J. F. Martin, “Hippo signaling impedes adult heart regeneration,” Development 140(23), 4683–4690 (2013). [CrossRef] [PubMed]

4.

M. Xin, Y. Kim, L. B. Sutherland, M. Murakami, X. Qi, J. McAnally, E. R. Porrello, A. I. Mahmoud, W. Tan, J. M. Shelton, J. A. Richardson, H. A. Sadek, R. Bassel-Duby, and E. N. Olson, “Hippo pathway effector Yap promotes cardiac regeneration,” Proc. Natl. Acad. Sci. U.S.A. 110(34), 13839–13844 (2013). [CrossRef] [PubMed]

5.

H. Jawad, A. R. Lyon, S. E. Harding, N. N. Ali, and A. R. Boccaccini, “Myocardial tissue engineering,” Br. Med. Bull. 87(1), 31–47 (2008). [CrossRef] [PubMed]

6.

R. M. Setser, N. G. Smedira, M. L. Lieber, E. D. Sabo, and R. D. White, “Left ventricular torsional mechanics after left ventricular reconstruction surgery for ischemic cardiomyopathy,” J. Thorac. Cardiovasc. Surg. 134(4), 888–896 (2007). [CrossRef] [PubMed]

7.

T. G. Kuznetsova, M. N. Starodubtseva, N. I. Yegorenkov, S. A. Chizhik, and R. I. Zhdanov, “Atomic force microscopy probing of cell elasticity,” Micron 38(8), 824–833 (2007). [CrossRef] [PubMed]

8.

A. B. Mathur, A. M. Collinsworth, W. M. Reichert, W. E. Kraus, and G. A. Truskey, “Endothelial, cardiac muscle and skeletal muscle exhibit different viscous and elastic properties as determined by atomic force microscopy,” J. Biomech. 34(12), 1545–1553 (2001). [CrossRef] [PubMed]

9.

A. P. Sarvazyan, O. V. Rudenko, S. D. Swanson, J. B. Fowlkes, and S. Y. Emelianov, “Shear wave elasticity imaging: a new ultrasonic technology of medical diagnostics,” Ultrasound Med. Biol. 24(9), 1419–1435 (1998). [CrossRef] [PubMed]

10.

Y. K. Mariappan, K. J. Glaser, and R. L. Ehman, “Magnetic resonance elastography: a review,” Clin. Anat. 23(5), 497–511 (2010). [CrossRef] [PubMed]

11.

J. Schmitt, “OCT elastography: imaging microscopic deformation and strain of tissue,” Opt. Express 3(6), 199–211 (1998). [CrossRef] [PubMed]

12.

C. Li, G. Guan, X. Cheng, Z. Huang, and R. K. Wang, “Quantitative elastography provided by surface acoustic waves measured by phase-sensitive optical coherence tomography,” Opt. Lett. 37(4), 722–724 (2012). [CrossRef] [PubMed]

13.

S. G. Adie, X. Liang, B. F. Kennedy, R. John, D. D. Sampson, and S. A. Boppart, “Spectroscopic optical coherence elastography,” Opt. Express 18(25), 25519–25534 (2010). [CrossRef] [PubMed]

14.

V. Crecea, A. Ahmad, and S. A. Boppart, “Magnetomotive optical coherence elastography for microrheology of biological tissues,” J. Biomed. Opt. 18(12), 121504 (2013). [CrossRef] [PubMed]

15.

K. M. Kennedy, B. F. Kennedy, R. A. McLaughlin, and D. D. Sampson, “Needle optical coherence elastography for tissue boundary detection,” Opt. Lett. 37(12), 2310–2312 (2012). [CrossRef] [PubMed]

16.

A. Nahas, M. Bauer, S. Roux, and A. C. Boccara, “3D static elastography at the micrometer scale using Full Field OCT,” Biomed. Opt. Express 4(10), 2138–2149 (2013). [CrossRef] [PubMed]

17.

X. Liang, A. L. Oldenburg, V. Crecea, E. J. Chaney, and S. A. Boppart, “Optical micro-scale mapping of dynamic biomechanical tissue properties,” Opt. Express 16(15), 11052–11065 (2008). [CrossRef] [PubMed]

18.

B. F. Kennedy, X. Liang, S. G. Adie, D. K. Gerstmann, B. C. Quirk, S. A. Boppart, and D. D. Sampson, “In vivo three-dimensional optical coherence elastography,” Opt. Express 19(7), 6623–6634 (2011). [CrossRef] [PubMed]

19.

W. Qi, R. Chen, L. Chou, G. Liu, J. Zhang, Q. Zhou, and Z. Chen, “Phase-resolved acoustic radiation force optical coherence elastography,” J. Biomed. Opt. 17(11), 110505 (2012). [CrossRef] [PubMed]

20.

X. Liang, S. G. Adie, R. John, and S. A. Boppart, “Dynamic spectral-domain optical coherence elastography for tissue characterization,” Opt. Express 18(13), 14183–14190 (2010). [CrossRef] [PubMed]

21.

B. F. Kennedy, K. M. Kennedy, and D. D. Sampson, “A Review of Optical Coherence Elastography: Fundamentals, Techniques and Prospects,” IEEE J. Sel. Top. Quantum Electron. 20(2), 1–17 (2014). [CrossRef]

22.

C. Sun, B. Standish, and V. X. D. Yang, “Optical coherence elastography: current status and future applications,” J. Biomed. Opt. 16(4), 043001 (2011). [CrossRef] [PubMed]

23.

X. Liang, V. Crecea, and S. A. Boppart, “Dynamic optical coherence elastography: a review,” J. Innov. Opt. Health Sci. 3(4), 221–233 (2010). [CrossRef] [PubMed]

24.

K. M. Kennedy, C. Ford, B. F. Kennedy, M. B. Bush, and D. D. Sampson, “Analysis of mechanical contrast in optical coherence elastography,” J. Biomed. Opt. 18(12), 121508 (2013). [CrossRef] [PubMed]

25.

B. F. Kennedy, T. R. Hillman, R. A. McLaughlin, B. C. Quirk, and D. D. Sampson, “In vivo dynamic optical coherence elastography using a ring actuator,” Opt. Express 17(24), 21762–21772 (2009). [CrossRef] [PubMed]

26.

S. G. Adie, B. F. Kennedy, J. J. Armstrong, S. A. Alexandrov, and D. D. Sampson, “Audio frequency in vivo optical coherence elastography,” Phys. Med. Biol. 54(10), 3129–3139 (2009). [CrossRef] [PubMed]

27.

A. L. Oldenburg and S. A. Boppart, “Resonant acoustic spectroscopy of soft tissues using embedded magnetomotive nanotransducers and optical coherence tomography,” Phys. Med. Biol. 55(4), 1189–1201 (2010). [CrossRef] [PubMed]

28.

C. Li, Z. Huang, and R. K. Wang, “Elastic properties of soft tissue-mimicking phantoms assessed by combined use of laser ultrasonics and low coherence interferometry,” Opt. Express 19(11), 10153–10163 (2011). [CrossRef] [PubMed]

29.

M. Razani, A. Mariampillai, C. Sun, T. W. H. Luk, V. X. D. Yang, and M. C. Kolios, “Feasibility of optical coherence elastography measurements of shear wave propagation in homogeneous tissue equivalent phantoms,” Biomed. Opt. Express 3(5), 972–980 (2012). [CrossRef] [PubMed]

30.

T. M. Nguyen, S. Song, B. Arnal, E. Y. Wong, Z. Huang, R. K. Wang, and M. O’Donnell, “Shear wave pulse compression for dynamic elastography using phase-sensitive optical coherence tomography,” J. Biomed. Opt. 19(1), 016013 (2014). [CrossRef] [PubMed]

31.

S. Song, Z. Huang, T. M. Nguyen, E. Y. Wong, B. Arnal, M. O’Donnell, and R. K. Wang, “Shear modulus imaging by direct visualization of propagating shear waves with phase-sensitive optical coherence tomography,” J. Biomed. Opt. 18(12), 121509 (2013). [CrossRef] [PubMed]

32.

T.-M. Nguyen, S. Song, B. Arnal, Z. Huang, M. O’Donnell, and R. K. Wang, “Visualizing ultrasonically induced shear wave propagation using phase-sensitive optical coherence tomography for dynamic elastography,” Opt. Lett. 39(4), 838–841 (2014). [CrossRef] [PubMed]

33.

C. Li, G. Guan, Z. Huang, M. Johnstone, and R. K. Wang, “Noncontact all-optical measurement of corneal elasticity,” Opt. Lett. 37(10), 1625–1627 (2012). [CrossRef] [PubMed]

34.

C. Li, G. Guan, R. Reif, Z. Huang, and R. K. Wang, “Determining elastic properties of skin by measuring surface waves from an impulse mechanical stimulus using phase-sensitive optical coherence tomography,” J. R. Soc. Interface 9(70), 831–841 (2012). [CrossRef] [PubMed]

35.

X. Liang and S. A. Boppart, “Biomechanical properties of In vivo human skin from dynamic optical coherence elastography,” IEEE Trans. Biomed. Eng. 57(4), 953–959 (2010). [CrossRef] [PubMed]

36.

S. Wang, J. Li, R. K. Manapuram, F. M. Menodiado, D. R. Ingram, M. D. Twa, A. J. Lazar, D. C. Lev, R. E. Pollock, and K. V. Larin, “Noncontact measurement of elasticity for the detection of soft-tissue tumors using phase-sensitive optical coherence tomography combined with a focused air-puff system,” Opt. Lett. 37(24), 5184–5186 (2012). [CrossRef] [PubMed]

37.

S. Wang and K. V. Larin, “Shear wave imaging optical coherence tomography (SWI-OCT) for ocular tissue biomechanics,” Opt. Lett. 39(1), 41–44 (2014). [CrossRef] [PubMed]

38.

S. Wang, K. V. Larin, J. Li, S. Vantipalli, R. K. Manapuram, S. Aglyamov, S. Emelianov, and M. D. Twa, “A focused air-pulse system for optical-coherence-tomography-based measurements of tissue elasticity,” Laser Phys. Lett. 10(7), 075605 (2013). [CrossRef]

39.

S. Song, Z. Huang, and R. K. Wang, “Tracking mechanical wave propagation within tissue using phase-sensitive optical coherence tomography: motion artifact and its compensation,” J. Biomed. Opt. 18(12), 121505 (2013). [CrossRef] [PubMed]

40.

J. Bercoff, M. Tanter, and M. Fink, “Supersonic shear imaging: a new technique for soft tissue elasticity mapping,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control 51(4), 396–409 (2004). [CrossRef] [PubMed]

41.

P. N. Wells and H. D. Liang, “Medical ultrasound: imaging of soft tissue strain and elasticity,” J. R. Soc. Interface 8(64), 1521–1549 (2011). [CrossRef] [PubMed]

42.

A. Biernacka and N. G. Frangogiannis, “Aging and Cardiac Fibrosis,” Aging Dis. 2(2), 158–173 (2011). [PubMed]

43.

M. Fink and M. Tanter, “Multiwave imaging and super resolution,” Print edition 63, 28–33 (2010).

44.

L. An, P. Li, T. T. Shen, and R. Wang, “High speed spectral domain optical coherence tomography for retinal imaging at 500,000 A‑lines per second,” Biomed. Opt. Express 2(10), 2770–2783 (2011). [CrossRef] [PubMed]

45.

M. A. Choma, A. K. Ellerbee, C. Yang, T. L. Creazzo, and J. A. Izatt, “Spectral-domain phase microscopy,” Opt. Lett. 30(10), 1162–1164 (2005). [CrossRef] [PubMed]

46.

S. Chen, M. W. Urban, C. Pislaru, R. Kinnick, Y. Zheng, A. Yao, and J. F. Greenleaf, “Shearwave dispersion ultrasound vibrometry (SDUV) for measuring tissue elasticity and viscosity,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control 56(1), 55–62 (2009). [CrossRef] [PubMed]

47.

W. Hiesinger, M. J. Brukman, R. C. McCormick, J. R. Fitzpatrick 3rd, J. R. Frederick, E. C. Yang, J. R. Muenzer, N. A. Marotta, M. F. Berry, P. Atluri, and Y. J. Woo, “Myocardial tissue elastic properties determined by atomic force microscopy after stromal cell-derived factor 1α angiogenic therapy for acute myocardial infarction in a murine model,” J. Thorac. Cardiovasc. Surg. 143(4), 962–966 (2012). [CrossRef] [PubMed]

48.

R. C. Chivers, “Tissue characterization,” Ultrasound Med. Biol. 7(1), 1–20 (1981). [CrossRef] [PubMed]

49.

B. R. Klyen, L. Scolaro, T. Shavlakadze, M. D. Grounds, and D. D. Sampson, “Optical coherence tomography can assess skeletal muscle tissue from mouse models of muscular dystrophy by parametric imaging of the attenuation coefficient,” Biomed. Opt. Express 5(4), 1217–1232 (2014). [CrossRef] [PubMed]

50.

C. Fan and G. Yao, “Imaging myocardial fiber orientation using polarization sensitive optical coherence tomography,” Biomed. Opt. Express 4(3), 460–465 (2013). [CrossRef] [PubMed]

51.

M. Razani, T. W. H. Luk, A. Mariampillai, P. Siegler, T.-R. Kiehl, M. C. Kolios, and V. X. D. Yang, “Optical coherence tomography detection of shear wave propagation in inhomogeneous tissue equivalent phantoms and ex-vivo carotid artery samples,” Biomed. Opt. Express 5(3), 895–906 (2014). [CrossRef] [PubMed]

52.

D. G. Melrose, B. Dreyer, H. H. Bentall, and J. B. E. Baker, “Elective Cardiac Arrest,” Lancet 266(6879), 21–23 (1955). [CrossRef] [PubMed]

53.

I. V. Larina, K. V. Larin, M. E. Dickinson, and M. Liebling, “Sequential Turning Acquisition and Reconstruction (STAR) method for four-dimensional imaging of cyclically moving structures,” Biomed. Opt. Express 3(3), 650–660 (2012). [CrossRef] [PubMed]

54.

M. Liebling, A. S. Forouhar, M. Gharib, S. E. Fraser, and M. E. Dickinson, “Four-dimensional cardiac imaging in living embryos via postacquisition synchronization of nongated slice sequences,” J. Biomed. Opt. 10(5), 054001 (2005). [CrossRef] [PubMed]

55.

M. W. Jenkins, Y. T. Wang, Y. Q. Doughman, M. Watanabe, Y. Cheng, and A. M. Rollins, “Optical pacing of the adult rabbit heart,” Biomed. Opt. Express 4(9), 1626–1635 (2013). [CrossRef] [PubMed]

56.

J. Xia, R. D. Miller, and C. B. Park, “Estimation of near-surface shear-wave velocity by inversion of Rayleigh waves,” Geophysics 64(3), 691–700 (1999). [CrossRef]

OCIS Codes
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Optical Coherence Tomography

History
Original Manuscript: April 15, 2014
Revised Manuscript: May 21, 2014
Manuscript Accepted: May 23, 2014
Published: May 30, 2014

Citation
Shang Wang, Andrew L. Lopez, Yuka Morikawa, Ge Tao, Jiasong Li, Irina V. Larina, James F. Martin, and Kirill V. Larin, "Noncontact quantitative biomechanical characterization of cardiac muscle using shear wave imaging optical coherence tomography," Biomed. Opt. Express 5, 1980-1992 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-7-1980


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. S. L. Murphy, J. Xu, and K. D. Kochanek, “Deaths: Final Data for 2010,” Natl. Vital Stat. Rep.61, 1–117 (2013).
  2. M. Mercola, P. Ruiz-Lozano, and M. D. Schneider, “Cardiac muscle regeneration: lessons from development,” Genes Dev.25(4), 299–309 (2011). [CrossRef] [PubMed]
  3. T. Heallen, Y. Morikawa, J. Leach, G. Tao, J. T. Willerson, R. L. Johnson, and J. F. Martin, “Hippo signaling impedes adult heart regeneration,” Development140(23), 4683–4690 (2013). [CrossRef] [PubMed]
  4. M. Xin, Y. Kim, L. B. Sutherland, M. Murakami, X. Qi, J. McAnally, E. R. Porrello, A. I. Mahmoud, W. Tan, J. M. Shelton, J. A. Richardson, H. A. Sadek, R. Bassel-Duby, and E. N. Olson, “Hippo pathway effector Yap promotes cardiac regeneration,” Proc. Natl. Acad. Sci. U.S.A.110(34), 13839–13844 (2013). [CrossRef] [PubMed]
  5. H. Jawad, A. R. Lyon, S. E. Harding, N. N. Ali, and A. R. Boccaccini, “Myocardial tissue engineering,” Br. Med. Bull.87(1), 31–47 (2008). [CrossRef] [PubMed]
  6. R. M. Setser, N. G. Smedira, M. L. Lieber, E. D. Sabo, and R. D. White, “Left ventricular torsional mechanics after left ventricular reconstruction surgery for ischemic cardiomyopathy,” J. Thorac. Cardiovasc. Surg.134(4), 888–896 (2007). [CrossRef] [PubMed]
  7. T. G. Kuznetsova, M. N. Starodubtseva, N. I. Yegorenkov, S. A. Chizhik, and R. I. Zhdanov, “Atomic force microscopy probing of cell elasticity,” Micron38(8), 824–833 (2007). [CrossRef] [PubMed]
  8. A. B. Mathur, A. M. Collinsworth, W. M. Reichert, W. E. Kraus, and G. A. Truskey, “Endothelial, cardiac muscle and skeletal muscle exhibit different viscous and elastic properties as determined by atomic force microscopy,” J. Biomech.34(12), 1545–1553 (2001). [CrossRef] [PubMed]
  9. A. P. Sarvazyan, O. V. Rudenko, S. D. Swanson, J. B. Fowlkes, and S. Y. Emelianov, “Shear wave elasticity imaging: a new ultrasonic technology of medical diagnostics,” Ultrasound Med. Biol.24(9), 1419–1435 (1998). [CrossRef] [PubMed]
  10. Y. K. Mariappan, K. J. Glaser, and R. L. Ehman, “Magnetic resonance elastography: a review,” Clin. Anat.23(5), 497–511 (2010). [CrossRef] [PubMed]
  11. J. Schmitt, “OCT elastography: imaging microscopic deformation and strain of tissue,” Opt. Express3(6), 199–211 (1998). [CrossRef] [PubMed]
  12. C. Li, G. Guan, X. Cheng, Z. Huang, and R. K. Wang, “Quantitative elastography provided by surface acoustic waves measured by phase-sensitive optical coherence tomography,” Opt. Lett.37(4), 722–724 (2012). [CrossRef] [PubMed]
  13. S. G. Adie, X. Liang, B. F. Kennedy, R. John, D. D. Sampson, and S. A. Boppart, “Spectroscopic optical coherence elastography,” Opt. Express18(25), 25519–25534 (2010). [CrossRef] [PubMed]
  14. V. Crecea, A. Ahmad, and S. A. Boppart, “Magnetomotive optical coherence elastography for microrheology of biological tissues,” J. Biomed. Opt.18(12), 121504 (2013). [CrossRef] [PubMed]
  15. K. M. Kennedy, B. F. Kennedy, R. A. McLaughlin, and D. D. Sampson, “Needle optical coherence elastography for tissue boundary detection,” Opt. Lett.37(12), 2310–2312 (2012). [CrossRef] [PubMed]
  16. A. Nahas, M. Bauer, S. Roux, and A. C. Boccara, “3D static elastography at the micrometer scale using Full Field OCT,” Biomed. Opt. Express4(10), 2138–2149 (2013). [CrossRef] [PubMed]
  17. X. Liang, A. L. Oldenburg, V. Crecea, E. J. Chaney, and S. A. Boppart, “Optical micro-scale mapping of dynamic biomechanical tissue properties,” Opt. Express16(15), 11052–11065 (2008). [CrossRef] [PubMed]
  18. B. F. Kennedy, X. Liang, S. G. Adie, D. K. Gerstmann, B. C. Quirk, S. A. Boppart, and D. D. Sampson, “In vivo three-dimensional optical coherence elastography,” Opt. Express19(7), 6623–6634 (2011). [CrossRef] [PubMed]
  19. W. Qi, R. Chen, L. Chou, G. Liu, J. Zhang, Q. Zhou, and Z. Chen, “Phase-resolved acoustic radiation force optical coherence elastography,” J. Biomed. Opt.17(11), 110505 (2012). [CrossRef] [PubMed]
  20. X. Liang, S. G. Adie, R. John, and S. A. Boppart, “Dynamic spectral-domain optical coherence elastography for tissue characterization,” Opt. Express18(13), 14183–14190 (2010). [CrossRef] [PubMed]
  21. B. F. Kennedy, K. M. Kennedy, and D. D. Sampson, “A Review of Optical Coherence Elastography: Fundamentals, Techniques and Prospects,” IEEE J. Sel. Top. Quantum Electron.20(2), 1–17 (2014). [CrossRef]
  22. C. Sun, B. Standish, and V. X. D. Yang, “Optical coherence elastography: current status and future applications,” J. Biomed. Opt.16(4), 043001 (2011). [CrossRef] [PubMed]
  23. X. Liang, V. Crecea, and S. A. Boppart, “Dynamic optical coherence elastography: a review,” J. Innov. Opt. Health Sci.3(4), 221–233 (2010). [CrossRef] [PubMed]
  24. K. M. Kennedy, C. Ford, B. F. Kennedy, M. B. Bush, and D. D. Sampson, “Analysis of mechanical contrast in optical coherence elastography,” J. Biomed. Opt.18(12), 121508 (2013). [CrossRef] [PubMed]
  25. B. F. Kennedy, T. R. Hillman, R. A. McLaughlin, B. C. Quirk, and D. D. Sampson, “In vivo dynamic optical coherence elastography using a ring actuator,” Opt. Express17(24), 21762–21772 (2009). [CrossRef] [PubMed]
  26. S. G. Adie, B. F. Kennedy, J. J. Armstrong, S. A. Alexandrov, and D. D. Sampson, “Audio frequency in vivo optical coherence elastography,” Phys. Med. Biol.54(10), 3129–3139 (2009). [CrossRef] [PubMed]
  27. A. L. Oldenburg and S. A. Boppart, “Resonant acoustic spectroscopy of soft tissues using embedded magnetomotive nanotransducers and optical coherence tomography,” Phys. Med. Biol.55(4), 1189–1201 (2010). [CrossRef] [PubMed]
  28. C. Li, Z. Huang, and R. K. Wang, “Elastic properties of soft tissue-mimicking phantoms assessed by combined use of laser ultrasonics and low coherence interferometry,” Opt. Express19(11), 10153–10163 (2011). [CrossRef] [PubMed]
  29. M. Razani, A. Mariampillai, C. Sun, T. W. H. Luk, V. X. D. Yang, and M. C. Kolios, “Feasibility of optical coherence elastography measurements of shear wave propagation in homogeneous tissue equivalent phantoms,” Biomed. Opt. Express3(5), 972–980 (2012). [CrossRef] [PubMed]
  30. T. M. Nguyen, S. Song, B. Arnal, E. Y. Wong, Z. Huang, R. K. Wang, and M. O’Donnell, “Shear wave pulse compression for dynamic elastography using phase-sensitive optical coherence tomography,” J. Biomed. Opt.19(1), 016013 (2014). [CrossRef] [PubMed]
  31. S. Song, Z. Huang, T. M. Nguyen, E. Y. Wong, B. Arnal, M. O’Donnell, and R. K. Wang, “Shear modulus imaging by direct visualization of propagating shear waves with phase-sensitive optical coherence tomography,” J. Biomed. Opt.18(12), 121509 (2013). [CrossRef] [PubMed]
  32. T.-M. Nguyen, S. Song, B. Arnal, Z. Huang, M. O’Donnell, and R. K. Wang, “Visualizing ultrasonically induced shear wave propagation using phase-sensitive optical coherence tomography for dynamic elastography,” Opt. Lett.39(4), 838–841 (2014). [CrossRef] [PubMed]
  33. C. Li, G. Guan, Z. Huang, M. Johnstone, and R. K. Wang, “Noncontact all-optical measurement of corneal elasticity,” Opt. Lett.37(10), 1625–1627 (2012). [CrossRef] [PubMed]
  34. C. Li, G. Guan, R. Reif, Z. Huang, and R. K. Wang, “Determining elastic properties of skin by measuring surface waves from an impulse mechanical stimulus using phase-sensitive optical coherence tomography,” J. R. Soc. Interface9(70), 831–841 (2012). [CrossRef] [PubMed]
  35. X. Liang and S. A. Boppart, “Biomechanical properties of In vivo human skin from dynamic optical coherence elastography,” IEEE Trans. Biomed. Eng.57(4), 953–959 (2010). [CrossRef] [PubMed]
  36. S. Wang, J. Li, R. K. Manapuram, F. M. Menodiado, D. R. Ingram, M. D. Twa, A. J. Lazar, D. C. Lev, R. E. Pollock, and K. V. Larin, “Noncontact measurement of elasticity for the detection of soft-tissue tumors using phase-sensitive optical coherence tomography combined with a focused air-puff system,” Opt. Lett.37(24), 5184–5186 (2012). [CrossRef] [PubMed]
  37. S. Wang and K. V. Larin, “Shear wave imaging optical coherence tomography (SWI-OCT) for ocular tissue biomechanics,” Opt. Lett.39(1), 41–44 (2014). [CrossRef] [PubMed]
  38. S. Wang, K. V. Larin, J. Li, S. Vantipalli, R. K. Manapuram, S. Aglyamov, S. Emelianov, and M. D. Twa, “A focused air-pulse system for optical-coherence-tomography-based measurements of tissue elasticity,” Laser Phys. Lett.10(7), 075605 (2013). [CrossRef]
  39. S. Song, Z. Huang, and R. K. Wang, “Tracking mechanical wave propagation within tissue using phase-sensitive optical coherence tomography: motion artifact and its compensation,” J. Biomed. Opt.18(12), 121505 (2013). [CrossRef] [PubMed]
  40. J. Bercoff, M. Tanter, and M. Fink, “Supersonic shear imaging: a new technique for soft tissue elasticity mapping,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control51(4), 396–409 (2004). [CrossRef] [PubMed]
  41. P. N. Wells and H. D. Liang, “Medical ultrasound: imaging of soft tissue strain and elasticity,” J. R. Soc. Interface8(64), 1521–1549 (2011). [CrossRef] [PubMed]
  42. A. Biernacka and N. G. Frangogiannis, “Aging and Cardiac Fibrosis,” Aging Dis.2(2), 158–173 (2011). [PubMed]
  43. M. Fink and M. Tanter, “Multiwave imaging and super resolution,” Print edition 63, 28–33 (2010).
  44. L. An, P. Li, T. T. Shen, and R. Wang, “High speed spectral domain optical coherence tomography for retinal imaging at 500,000 A‑lines per second,” Biomed. Opt. Express2(10), 2770–2783 (2011). [CrossRef] [PubMed]
  45. M. A. Choma, A. K. Ellerbee, C. Yang, T. L. Creazzo, and J. A. Izatt, “Spectral-domain phase microscopy,” Opt. Lett.30(10), 1162–1164 (2005). [CrossRef] [PubMed]
  46. S. Chen, M. W. Urban, C. Pislaru, R. Kinnick, Y. Zheng, A. Yao, and J. F. Greenleaf, “Shearwave dispersion ultrasound vibrometry (SDUV) for measuring tissue elasticity and viscosity,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control56(1), 55–62 (2009). [CrossRef] [PubMed]
  47. W. Hiesinger, M. J. Brukman, R. C. McCormick, J. R. Fitzpatrick, J. R. Frederick, E. C. Yang, J. R. Muenzer, N. A. Marotta, M. F. Berry, P. Atluri, and Y. J. Woo, “Myocardial tissue elastic properties determined by atomic force microscopy after stromal cell-derived factor 1α angiogenic therapy for acute myocardial infarction in a murine model,” J. Thorac. Cardiovasc. Surg.143(4), 962–966 (2012). [CrossRef] [PubMed]
  48. R. C. Chivers, “Tissue characterization,” Ultrasound Med. Biol.7(1), 1–20 (1981). [CrossRef] [PubMed]
  49. B. R. Klyen, L. Scolaro, T. Shavlakadze, M. D. Grounds, and D. D. Sampson, “Optical coherence tomography can assess skeletal muscle tissue from mouse models of muscular dystrophy by parametric imaging of the attenuation coefficient,” Biomed. Opt. Express5(4), 1217–1232 (2014). [CrossRef] [PubMed]
  50. C. Fan and G. Yao, “Imaging myocardial fiber orientation using polarization sensitive optical coherence tomography,” Biomed. Opt. Express4(3), 460–465 (2013). [CrossRef] [PubMed]
  51. M. Razani, T. W. H. Luk, A. Mariampillai, P. Siegler, T.-R. Kiehl, M. C. Kolios, and V. X. D. Yang, “Optical coherence tomography detection of shear wave propagation in inhomogeneous tissue equivalent phantoms and ex-vivo carotid artery samples,” Biomed. Opt. Express5(3), 895–906 (2014). [CrossRef] [PubMed]
  52. D. G. Melrose, B. Dreyer, H. H. Bentall, and J. B. E. Baker, “Elective Cardiac Arrest,” Lancet266(6879), 21–23 (1955). [CrossRef] [PubMed]
  53. I. V. Larina, K. V. Larin, M. E. Dickinson, and M. Liebling, “Sequential Turning Acquisition and Reconstruction (STAR) method for four-dimensional imaging of cyclically moving structures,” Biomed. Opt. Express3(3), 650–660 (2012). [CrossRef] [PubMed]
  54. M. Liebling, A. S. Forouhar, M. Gharib, S. E. Fraser, and M. E. Dickinson, “Four-dimensional cardiac imaging in living embryos via postacquisition synchronization of nongated slice sequences,” J. Biomed. Opt.10(5), 054001 (2005). [CrossRef] [PubMed]
  55. M. W. Jenkins, Y. T. Wang, Y. Q. Doughman, M. Watanabe, Y. Cheng, and A. M. Rollins, “Optical pacing of the adult rabbit heart,” Biomed. Opt. Express4(9), 1626–1635 (2013). [CrossRef] [PubMed]
  56. J. Xia, R. D. Miller, and C. B. Park, “Estimation of near-surface shear-wave velocity by inversion of Rayleigh waves,” Geophysics64(3), 691–700 (1999). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Supplementary Material


» Media 1: MP4 (1929 KB)     

Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited