Measurement of interaction force between RGD-peptide and Hela cell surface by optical tweezers
Chinese Optics Letters, Vol. 10, Issue 10, pp. 101701-101701 (2012)
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Abstract
Since RGD peptides (R: arginine; G: glycine; D: aspartic acid) are found to promote cell adhesion, they are modified at numerous materials surface for medical applications such as drug delivery and regenerative medicine. Peptide-cell surface interactions play a key role in the above applications. In this letter, we study the adhesion force between the RGD-coated bead and Hela cell surface by optical tweezes. The adhesion is dominated by the binding of α5β1 and RGD-peptide with higher adhesion probability and stronger adhesion strength compared with the adhesion of bare bead and cell surface. The binding force for a single α5β1-GRGDSP pair is determined to be 16.8 pN at a loading rate of 1.5 nN/s. The unstressed off-rate is 1.65 × 10-2 s-1 and the distance of transition state for the rigid binding model is 3.0 nm.
© 2012 Chinese Optics Letters
OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(350.4855) Other areas of optics : Optical tweezers or optical manipulation
ToC Category:
Medical Optics and Biotechnology
Citation
Mincheng Zhong, Guosheng Xue, Jinhua Zhou, Ziqiang Wang, and Yinmei Li, "Measurement of interaction force between RGD-peptide and Hela cell surface by
optical tweezers," Chin. Opt. Lett. 10, 101701-101701 (2012)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-10-10-101701
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