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Chinese Optics Letters

Chinese Optics Letters


  • Editor: Zhizhan Xu
  • Vol. 11, Iss. 9 — Sep. 1, 2013
  • pp: 091701–

Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells

Jie Yu, Xunliang Tong, Chengbin Li, Yining Huang, and Anpei Ye  »View Author Affiliations

Chinese Optics Letters, Vol. 11, Issue 9, pp. 091701- (2013)

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We carry out in situ single-molecule measurements of the specific interaction between apolipoprotein A-I (apoA-I) and ATP binding cassette transporter A1 (ABCA1) on THP-1 cells. Single-molecule force spectroscopy shows that similar to normal apoA-I, the dysfunctional apoA-I from diabetes patients interacts with ABCA1 via two different binding sites on the cells. The strength of dysfunctional apoA-I binding to a high-capacity binding site is 26.5+(-)4.9 pN. The minor direct apoA-I/ABCA1 binding strength is 56.7+(-)4.1 pN. These results facilitate a pathological understanding of the mechanisms that underlie the specific interaction of apoA-I and ABCA1 at the single-molecule level.

© 2013 Chinese Optics Letters

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(140.7010) Lasers and laser optics : Laser trapping
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

Jie Yu, Xunliang Tong, Chengbin Li, Yining Huang, and Anpei Ye, "Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells," Chin. Opt. Lett. 11, 091701- (2013)

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