We carry out in situ single-molecule measurements of the specific interaction between apolipoprotein A-I (apoA-I) and ATP binding cassette transporter A1 (ABCA1) on THP-1 cells. Single-molecule force spectroscopy shows that similar to normal apoA-I, the dysfunctional apoA-I from diabetes patients interacts with ABCA1 via two different binding sites on the cells. The strength of dysfunctional apoA-I binding to a high-capacity binding site is 26.5+(-)4.9 pN. The minor direct apoA-I/ABCA1 binding strength is 56.7+(-)4.1 pN. These results facilitate a pathological understanding of the mechanisms that underlie the specific interaction of apoA-I and ABCA1 at the single-molecule level.
© 2013 Chinese Optics Letters
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(140.7010) Lasers and laser optics : Laser trapping
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy
Medical Optics and Biotechnology
Jie Yu, Xunliang Tong, Chengbin Li, Yining Huang, and Anpei Ye, "Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells," Chin. Opt. Lett. 11, 091701- (2013)