OSA's Digital Library

Chinese Optics Letters

Chinese Optics Letters


  • Vol. 6, Iss. 12 — Dec. 1, 2008
  • pp: 947–949

Investigation of photodynamic therapy on streptococcus mutans of oral biofilm

Zhaohui Zou, Ping Gao, Huijuan Yin, and Yingxin Li  »View Author Affiliations

Chinese Optics Letters, Vol. 6, Issue 12, pp. 947-949 (2008)

View Full Text Article

Acrobat PDF (165 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

  • Export Citation/Save Click for help


We investigated the effect of photodynamic therapy (PDT) with hematoporphyrin monomethyl ether (HMME) on the viability of Streptococcus mutans (S. mutans) cells on biofilms in vitro. Streptococcus mutans is the primary etiological agent of human dental caries. Since dental caries are localized infections, such plaque-related diseases would be well suited to PDT. The diode laser used in this study had the wavelength of 635 nm, whose output power was 10 mW and the energy density was 12.74 J/cm2. HMME was used as photosensitizer. Samples were prepared and divided into five groups: (1) HMME; (2) Laser; (3) HMME+Laser; (4) Control group (+) with chlorhexidine; and (5) Control group (-) with sterile physiological saline. Inoculum of S. mutans incubated with HMME also examined with fluorescence microscopy. PDT exhibited a significantly (P<0.05) increased antimicrobial potential compared with 20 \mum/mL HMME only, laser only, 0.05% chlorhexidine, and 0.9% sterile physiological saline, which reduced the S. mutans of the biofilm most effectively. Laser and 0.05% chlorhexidine were caused reduction in the viable counts of S. mutans significantly different (P<0.05) also, but these two test treatments did not statistically differ from each other. HMME group did not statistically differ with negative control group. Fluorescence microscopy indicated that HMME localized primarily in the S. mutans of the biofilm. It was demonstrated that HMME-mediated PDT was efficient at killing S. mutans of biofilms and a useful approach in the treatment of dental plaque-related diseases.

© 2008 Chinese Optics Letters

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.5180) Medical optics and biotechnology : Photodynamic therapy

Zhaohui Zou, Ping Gao, Huijuan Yin, and Yingxin Li, "Investigation of photodynamic therapy on streptococcus mutans of oral biofilm," Chin. Opt. Lett. 6, 947-949 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. C. Robinson, J. Kirkham, R. Percival, R. C. Shore, W. A. Bonass, S. J. Brookes, L. Kusa, H. Nakagaki, K. Kato, and B. Nattress, Caries Res. 31, 194 (1997).
  2. P. Sreenivasan and A. Gaffar, J. Clin. Periodontol 29, 965 (2002).
  3. P. Meisel and T. Kocher, Journal of Photochemistry and Photobiology B 79, 159 (2005).
  4. P. Courvalin, Journal of Antimicrobial Chemotherapy 37, 855 (1996).
  5. T. P. Paulino, K. F. Ribeiro, G. Thedei, Jr., A. C. Tedesco, and P. Ciancaglini, Archives of Oral Biology 50, 353 (2005).
  6. I. M. Bevilacqua, R. A. Nicolau, S. Khouri, A. Brugnera, Jr., G. R. Teodoro, R. A. Zangaro, and M. T. T. Pacheco, Photomedicine and Laser Surgery 25, 513 (2007).
  7. J. W. Costerton, P. S. Stewart, and E. P. Greenberg, Science 284, 1318 (1999).
  8. S. Shani, M. Friedman, and D. Steinberg, Caries Res. 34, 260 (2000).
  9. M. R. Hamblin and T. Hasan, Photochemical and Photobiological Sciences 3, 436 (2004).
  10. K. Konopka and T. Goslinski, J. Dent. Res. 86, 694 (2007).

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited