Spectral imaging of time-resolved anisotropy: theory and experiment
Chinese Optics Letters, Vol. 8, Issue 10, pp. 937-939 (2010)
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Abstract
Time-resolved fluorescence anisotropy on the nanosecond time scale is useful for the study of the rapid rotation of macromolecules. A system combining the capabilities of fluorescence spectral imaging with time-resolved fluorescence anisotropy and enabling the wide-field measurement of the spectroscopic parameters of fluorophores is discussed. The phasor approach is used to quantitatively analyze the time-resolved fluorescence anisotropy by transforming the polarized parallel and perpendicular components to the phasor space in the frequency domain, respectively, and a unique way to calculate the fluorescence rotational correlation time is put forward. Experimental results prove that the phasor approach is a proper model for the time-resolved fluorescence anisotropy.
© 2010 Chinese Optics Letters
OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
Citation
Yanzhou Zhou, Qingruo Wang, Jingsong He, and Lerong Lin, "Spectral imaging of time-resolved anisotropy: theory and experiment," Chin. Opt. Lett. 8, 937-939 (2010)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-8-10-937
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