Abstract
Porous silicon (PS) suitable for optical detection of immunoreaction is
fabricated. The structure of immunosensor is prepared by the following steps:
oxidization, silanization, glutaraldehyde cross-linker, and covalent binding of
antibody. When antigen is added into the immunosensor, the Raman intensity is
estimated to be linearly reduced according to the concentration of the surface
protective antigen protein A (spaA) of below 4.0 ?g·ml?1. The ultimate detection
limit is 1.412×10^2 pg.ml^{?1}. Controlled experiments are also presented with
non-immune antigen of the spaA, and results show that the immunosensor has high
specificity. Compared with the conventional enzyme-linked immuno sorbent assay
(ELISA), this method is quick, inexpensive, and label-free.
© 2011 Chinese Optics Letters
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