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Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Editor: Zhizhan Xu
  • Vol. 10, Iss. 10 — Oct. 1, 2012
  • pp: 101701–101701

Measurement of interaction force between RGD-peptide and Hela cell surface by optical tweezers

Mincheng Zhong, Guosheng Xue, Jinhua Zhou, Ziqiang Wang, and Yinmei Li  »View Author Affiliations


Chinese Optics Letters, Vol. 10, Issue 10, pp. 101701-101701 (2012)


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Abstract

Since RGD peptides (R: arginine; G: glycine; D: aspartic acid) are found to promote cell adhesion, they are modified at numerous materials surface for medical applications such as drug delivery and regenerative medicine. Peptide-cell surface interactions play a key role in the above applications. In this letter, we study the adhesion force between the RGD-coated bead and Hela cell surface by optical tweezes. The adhesion is dominated by the binding of α5β1 and RGD-peptide with higher adhesion probability and stronger adhesion strength compared with the adhesion of bare bead and cell surface. The binding force for a single α5β1-GRGDSP pair is determined to be 16.8 pN at a loading rate of 1.5 nN/s. The unstressed off-rate is 1.65 × 10-2 s-1 and the distance of transition state for the rigid binding model is 3.0 nm.

© 2012 Chinese Optics Letters

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Medical Optics and Biotechnology

Citation
Mincheng Zhong, Guosheng Xue, Jinhua Zhou, Ziqiang Wang, and Yinmei Li, "Measurement of interaction force between RGD-peptide and Hela cell surface by optical tweezers," Chin. Opt. Lett. 10, 101701-101701 (2012)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-10-10-101701

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