The murine model of hindlimb ischemia is extensively used in studies on the physiology and pathology of ischemia and angiogenesis. Traditional non-invasive evaluation methods, such as laser or ultrasound blood flow perfusion imaging and micro-CT angiography, are limited either by low resolution or toxic exogenous agents. Relying on intrinsic high optical absorption contrast, we conduct label-free imaging of subcutaneous blood vasculature in the hindlimb of murine models by photoacoustic microscopy (PAM). The angiogenesis induced by ischemia in the hindlimb is successfully observed at high resolution in vivo and non-invasively. PAM is a potentially powerful imaging method for studying ischemic diseases and resultant angiogenesis.
© 2012 Chinese Optics Letters
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
Medical Optics and Biotechnology
Shuoqi Ye, Junyu Yang, Jianzhong Xi, Qiushi Ren, and Changhui Li, "Studying murine hindlimb ischemia by photoacoustic microscopy," Chin. Opt. Lett. 10, 121701-121701 (2012)